Fig. 6

Matrine combined with Osthole inhibits via the PERK pathway apoptosis in the cells transfected with Cap. After the PK-15 cells were transfected with p-Cap for 6 h, the cells were treated with Matrine and Osthole combination for 48 h and the samples were harvested. a and b The level of Cap protein in the group transfected with p-Cap was markedly elevated in the Western blotting. c-g As revealed by Western blotting, Matrine combined with Osthole down-regulated the marker protein GRP78 in the ER pathway, as well as the expression levels of the cleaved caspase-3 and Bcl-2 in the PERK apoptosis pathway. h-o Levels of apoptins in the PERK pathway were determined by Western blotting. Data are expressed in the form of mean ± SEM, with different letters (a, b, c, d, etc.) indicating a significant difference from other groups (p < 0.05)