Fig. 4
From: The combined usage of Matrine and Osthole inhibited endoplasmic reticulum apoptosis induced by PCV2
PCV2-induced PK-15 cell apoptosis inhibited by Matrine combined with Osthole. After the PK-15 cells were infected with 104.4 TCID50 of PCV2, the cell samples were harvested after 48 h incubation with Matrine combined with Osthole. a and b The apoptotic rate was analyzed by flow cytometry. Data indicate the sum of the late apoptosis of the right upper quadrant and the early apoptosis of the right lower quadrant. c and d The level of cleaved caspase-3 was down-regulated significantly in the Western blotting. e and f Changes in MMP after treatment for 48 h with a combination of drugs were analyzed by JC-1. R2 represents the changes in the MMP. g-k Expression of the key apoptins were analyzed by Western blotting and grayscale analysis. Data are expressed in the form of mean ± SEM, with different letters (a, ,b, c, d, etc.) indicating a significant difference from other groups (p < 0.05)