Fig. 3
From: Experimental determination of evolutionary barriers to horizontal gene transfer
Analyses of the selective barriers on HGT. The mean selective effects of 44 transferred S. Typhimurium genes expressed in E. coli background: a. divided into informational and operational genes based on functional categories; b. plotted against predicted number of PPIs; c. absolute deviation in %GC between orthologs; d. absolute deviation in codon usage between orthologs; e. gene length; f. number of disordered regions in the amino-acid sequences. All 5 factors given in a. to e. are used as explanatory variables in a multiple regression model (single p-values at the bottom right of each panel). e. We repeated the simple linear regression for the gene length as it was the only factor with a significant effect in the multiple regression. Black lines in e and f show the best fit for the simple linear regression between the two variables; gray dashed lines show the zero line