Fig. 3From: High-quality genome sequence assembly of R.A73 Enterococcus faecium isolated from freshwater fish mucusPhylogenetic tree based on 16S rDNA sequences. 16S rDNA sequences were downloaded from the National Center for Biotechnology Information (NCBI) database and aligned using Muscle [23] as part of the MEGA7 [24] software to generate 1000 bootstrap replicates followed by a search for the best-scoring Maximum Likelihood (ML) tree. The tree was saved in Newick format and displayed, manipulated, and annotated using iTOL 3 [25]Back to article page