Skip to main content
Fig. 8 | BMC Microbiology

Fig. 8

From: Short chain fatty acids induced the type 1 and type 2 fimbrillin-dependent and fimbrillin-independent initial attachment and colonization of Actinomyces oris monoculture but not coculture with streptococci

Fig. 8

Effects of anti-GroEL antibodies on the INAC of A. oris. TSB only (a, b, e and f) and with 60 mM butyric acid (c, d, g and h) were applied to the cultures for the INAC assays of the wild-type A. oris MG1 and the MG1 ΔfimA strains. Anti-GroEL rabbit antibodies were diluted 1/4000 and applied to the cultures (c, d, g and h). The attached and colonized cells were observed at the edges of the wells. INAC areas were observed by confocal microscopy. Merged images of the live cells (green colour) and dead cells (red colour) are presented in the pictures, in which the effects of butyric acid on the INAC are observed. All CLSM images were obtained with a 10× objective. Scale bars indicate 100 μm. Representative data from more than three independent experiments are presented in each panel

Back to article page