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Fig. 5 | BMC Microbiology

Fig. 5

From: Short chain fatty acids induced the type 1 and type 2 fimbrillin-dependent and fimbrillin-independent initial attachment and colonization of Actinomyces oris monoculture but not coculture with streptococci

Fig. 5

Effects of 60 mM butyric acid with lowered pH on INAC. a INAC of the wild-type A. oris MG1 and the MG1 ΔfimA mutant strains were observed under the control condition, 60 mM butyric acid condition, pH 4.7 condition prepared with HCl, pH 7.2 condition prepared with NaOH, 60 mM sodium butyrate condition and pH 4.7 condition with 60 mM sodium butyrate prepared with HCl. The attached and colonized cells were stained using a LIVE/DEAD staining kit and observed by CLSM at the edges of the wells. All CLSM images were obtained with a 10× objective. Scale bars indicate 100 μm. b Images were further analysed to determine the areas of live cells and dead cells in MGΔfimA using ImageJ. Representative data from more than three independent experiments are presented in each panel

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