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Fig. 4 | BMC Microbiology

Fig. 4

From: Short chain fatty acids induced the type 1 and type 2 fimbrillin-dependent and fimbrillin-independent initial attachment and colonization of Actinomyces oris monoculture but not coculture with streptococci

Fig. 4

Effects of 60 mM butyric acid and HCl-mediated pH conditions on INAC. a INAC of the wild-type A. oris MG1 and the MG1 ΔfimA mutant was observed in conditions with or without 60 mM butyric acid and at various pH values (pH 4.7, 5.0, 5.5 and 6.0) prepared with HCl. The attached and colonized cells were stained using a LIVE/DEAD staining kit and observed by CLSM at the edges of the wells. All CLSM images were obtained with a 10× objective. Scale bars indicate 100 μm. Images were further analysed to determine the areas of live cells and dead cells in MG1 using ImageJ. b Representative data from more than three independent experiments are presented in each panel

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BMC Microbiology

ISSN: 1471-2180

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