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Fig. 3 | BMC Microbiology

Fig. 3

From: Short chain fatty acids induced the type 1 and type 2 fimbrillin-dependent and fimbrillin-independent initial attachment and colonization of Actinomyces oris monoculture but not coculture with streptococci

Fig. 3

Effect of SCFAs on the INAC of the wild-type A. oris and the fimbrillin mutants. The INAC of the wild-type A. oris MG1 and the ΔfimP, ΔfimQ, ΔfimA and ΔfimB mutants were observed under conditions with or without SCFAs. The attached and colonized cells were stained using a LIVE/DEAD staining kit and observed by CLSM at the edges of the wells. All CLSM images were obtained with a 10× objective. TSB without SCFAs, TSB with 60 mM acetic acid, TSB with 60 mM butyric acid, TSB with 60 mM formic acid, TSB with 60 mM lactic acid, TSBs with 60 mM propionic acid and TSB with 60 mM valeric acid were used as the media for the INAC assays for the wild-type A. oris MG1 and the MG1 ΔfimA mutant strain. Live and dead cell areas were analysed by ImageJ from CLSM images, and the ratios of the live areas to the dead cell areas were calculated by ImageJ. The data represent the mean ± standard deviation (SD) of three independent experiments. The asterisks indicate a significant difference between the two groups (Student’s t-test, p-value < 0.05, *: no SCFAs vs. SCFAs, **: MG1 vs. each MG1 mutant)

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BMC Microbiology

ISSN: 1471-2180

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