Fig. 2

Effects of curcumin on biofilm activity of S. mutans in different groups at 5 min/24 h. Bacteria were inoculated in 96-well microtiter plates containing BHIS medium to form a 24-h biofilm. Supernatant was discard and it was washed with PBS for three times. The experimental group was added with 500 μM curcumin in BHIS, and the negative control group was only added with BHIS. After 5 min (a) and 24 h (b), the activity of the biofilm of the S. mutans was evaluated by MTT assay. Meanwhile compared the difference of reduction in biofilm viability (%) between the SECC group and CF group after 5 min and 24 h drug treatment (c). * indicates that the data is statistically different (* P < 0.05, ** P < 0.01, ***P < 0.001)