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Fig. 3 | BMC Microbiology

Fig. 3

From: Symbiotic microbiome Staphylococcus aureus from human nasal mucus modulates IL-33-mediated type 2 immune responses in allergic nasal mucosa

Fig. 3

Staphylococcus species suppress expression of interleukin-33 (IL-33). ARNE cells were inoculated with nasal commensal S. aureus (AR-SA) and S. epidermidis (AR-SE) from subjects with AR at a MOI of 0.25. Levels of mRNAs encoding epithelial cell-derived Th2 cytokines including IL-33 and thymic stromal lymphopoietin (TSLP) were monitored by real-time PCR (IL-33 mRNA in AR-SA-inoculated ARNE cells (a) and AR-SE-inoculated ARNE cells (b), TSLP mRNA in AR-SA-inoculated ARNE cells (c) and AR-SE-inoculated ARNE cells (d). IL-33 mRNA and protein levels of normal human nasal epithelial (NHNE, white bar) and ARNE cells (Black bar) inoculated with AR-SA were analyzed by real-time PCR (e) and Western blot (f), respectively. Secreted IL-33 protein levels of normal human nasal epithelial (NHNE, white bar) and ARNE cells (Black bar) inoculated with AR-SA were analyzed by ELISA (g) Results are presented as mean ± standard deviation (SD) from five independent experiments and western blot results is also representative of five independent experiments. *p < 0.05 compared with control ARNE cells (a, b, c, and d). *p < 0.05 comparing levels between NHNE and ARNE cells (e, g). The original, unprocessed versions of full-length gel/blot images are included in the online data supplement (Additional file 3: supplementary figure)

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