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Table 3 Comparison of the specificity of several PCR and real-time qPCR protocols

From: Development and comparative validation of genomic-driven PCR-based assays to detect Xanthomonas citri pv. citri in citrus plants

Assay X. citri pv. citri X. citri pv. aurantifolii (n = 5) X. euvesicatoria pv. citrumelonis (n = 2) Other X. citria pathovars (n = 9) Other Xanthomonasa species (n = 11) Saprophytic xanthomonadsb (n = 15)
A (n = 63) A* (n = 11) Aw (n = 4)      
PCR Jpth1/2 62c 11 4 5 0 7 1 0
PCR VM3/4 63 11 4 5 0 7 1 0
PCR XACF/R 63 11 4 0 0 4 0 0
PCR XCF/R 63 11 4 0 0 9 0 0
PCR XAC1051-F/R 63 11 4 0 0 0 0 0
qPCR XAC1051-2qPCR 63 11 4 0 0 1 0 0
qPCR J-Taqpth-qPCR 63 11 4 0 0 3 0 0
qPCR VM-Syb-qPCR 63 11 4 5 0 7 1 0
  1. a Isolated from Citrus spp. but not pathogenic to Citrus spp.
  2. b Isolated from Citrus spp.
  3. c Number of positive samples