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Table 1 Pattern of gene mutations in rpoB gene detected by Genotype MTBDRplus and DNA sequencing of the rpoB gene for pattern of resistance in MTB strains

From: Detection of mutations in the rpoB gene of rifampicin-resistant Mycobacterium tuberculosis strains inhibiting wild type probe hybridization in the MTBDR plus assay by DNA sequencing directly from clinical specimens

   

Resistance classification

Gene

Codons

Number by drug resistance category and rpoB gene loci

Gene region

Absent wild type probe

DNA sequencing results

rpoB

531 (14)

9, 1, 1, 1

531–533

WT8a

TCG-TGG (Ser-Trp), TCG-TGC (Ser-Cys), TCG-TAT (Ser-Tyr), TCG-TTT (Ser-Phe)

531&526

1

531–533

WT8a

TCG-TGG (Ser-Trp) & CAC-TAC (His-Tyr)

531&605

1

–

–

TCG-TTC (Ser-Phe) & TAC-GAC (Tyr-Asp)

526 (5)

1, 1

525–530

WT7

CAC-TGC (His-Cys), CAC-AAC (His-Asp)

526, 506&531

1

525–530

–

CAC-CTC (His-Leu), TTC-TTA (Phe-Leu) & TCG-TGG (Ser-Trp)

526&528

1

–

WT7a

CAC-CTC (His-Leu) & CGC-TGC (Arg-Cys)

526&506

1

–

WT7

CAC-CTC (His-Leu) & TTC-TTA (Phe-Leu)

516 (12)

1

513–517

WT3a

Ins CTT (Leu) b/w 514–515

–

1

 

WT3

GAC-TAC (Asp-Tyr)

–

3, 6

513–517 & 516–519

WT3&4

GAC-GTC (Asp-Val), GAC-TAC (Asp-Tyr)

509&516

1

506–509, 513–517 & 516–519

WT 1,3&4

AGC-ATC (Glu-His), GAC-TAC (Asp-Tyr)

516

1

–

WT4a

GAC-GTC (Asp-Val)

513 (4)

2,1,2

 

WT3&4

CAA-CCA (Gln-Pro), CAA-GAA (Gln-Glu), CAA-GTA (Gln-Val)

513&526

1, 1

–

WT3&4

CAA-CTA (Gln-Leu) & CAC-CAG (His-Gln)

 

1

–

WT3&4

Ins CTT (Leu) at 513–514

515&572

1

513–517

WT3a

ATG-CTG (Met-Leu) & ATC-TTC (Ile-Phe)

511 (3)

1, 2, 1

510–513

WT2

CTG-CCG (Leu-Pro), Ins CCG (Pro) at 511–513, Ins GAG (Glu) at 511–512

518 (3)

2

518–522

WT5

AAC-GAC (Asn-Asp)

–

1

518–522 & 522–525

WT5&6

Del AAC (Thr)

518

1

516–520

WT4

AAC-GAC (Asn-Asp)

  1. aindeterminate results
  2. Bold columns showing new mutations