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Table 1 Pattern of gene mutations in rpoB gene detected by Genotype MTBDRplus and DNA sequencing of the rpoB gene for pattern of resistance in MTB strains

From: Detection of mutations in the rpoB gene of rifampicin-resistant Mycobacterium tuberculosis strains inhibiting wild type probe hybridization in the MTBDR plus assay by DNA sequencing directly from clinical specimens

    Resistance classification
Gene Codons Number by drug resistance category and rpoB gene loci Gene region Absent wild type probe DNA sequencing results
rpoB 531 (14) 9, 1, 1, 1 531–533 WT8a TCG-TGG (Ser-Trp), TCG-TGC (Ser-Cys), TCG-TAT (Ser-Tyr), TCG-TTT (Ser-Phe)
531&526 1 531–533 WT8a TCG-TGG (Ser-Trp) & CAC-TAC (His-Tyr)
531&605 1 TCG-TTC (Ser-Phe) & TAC-GAC (Tyr-Asp)
526 (5) 1, 1 525–530 WT7 CAC-TGC (His-Cys), CAC-AAC (His-Asp)
526, 506&531 1 525–530 CAC-CTC (His-Leu), TTC-TTA (Phe-Leu) & TCG-TGG (Ser-Trp)
526&528 1 WT7a CAC-CTC (His-Leu) & CGC-TGC (Arg-Cys)
526&506 1 WT7 CAC-CTC (His-Leu) & TTC-TTA (Phe-Leu)
516 (12) 1 513–517 WT3a Ins CTT (Leu) b/w 514–515
1   WT3 GAC-TAC (Asp-Tyr)
3, 6 513–517 & 516–519 WT3&4 GAC-GTC (Asp-Val), GAC-TAC (Asp-Tyr)
509&516 1 506–509, 513–517 & 516–519 WT 1,3&4 AGC-ATC (Glu-His), GAC-TAC (Asp-Tyr)
516 1 WT4a GAC-GTC (Asp-Val)
513 (4) 2,1,2   WT3&4 CAA-CCA (Gln-Pro), CAA-GAA (Gln-Glu), CAA-GTA (Gln-Val)
513&526 1, 1 WT3&4 CAA-CTA (Gln-Leu) & CAC-CAG (His-Gln)
  1 WT3&4 Ins CTT (Leu) at 513–514
515&572 1 513–517 WT3a ATG-CTG (Met-Leu) & ATC-TTC (Ile-Phe)
511 (3) 1, 2, 1 510–513 WT2 CTG-CCG (Leu-Pro), Ins CCG (Pro) at 511–513, Ins GAG (Glu) at 511–512
518 (3) 2 518–522 WT5 AAC-GAC (Asn-Asp)
1 518–522 & 522–525 WT5&6 Del AAC (Thr)
518 1 516–520 WT4 AAC-GAC (Asn-Asp)
  1. aindeterminate results
  2. Bold columns showing new mutations