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Fig. 2 | BMC Microbiology

Fig. 2

From: Development and clinical application of a novel CRISPR-Cas12a based assay for the detection of African swine fever virus

Fig. 2

Screening for highly active crRNAs. A total of 19 crRNAs targeting the ASFV p72 gene were designed, these should work as a “universal” crRNA set for the detection of ASFV. Two ASFV positive blood (GD/GZ/0227, GD/GZ/0311) and two negative blood were used to test crRNA activity for 15 (a) and 30 (b) min. Fluorescence signal reflects the activation of individual crRNA, n = 3 technical replicates; bars represent mean ± SD

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BMC Microbiology

ISSN: 1471-2180

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