Fig. 8

Hematoxylin and eosin (H&E) stained sections of representative bone samples after day 15 post-infection. Bone infected with sham pins (control) or with the USA300 LAC, msaABCR mutant, or complementation strains (a) at low (left) and high (right) magnification. Blue arrows show the points of insertion of the K-wires, green arrows show osteoblast cells, red arrows show osteoclasts cells, yellow arrows show newly formed bones, and orange arrows show bone necrosis. Total histopathological scores of infected bone (b). Histopathological scores were assessed on the basis of intraosseous acute inflammation (IAI, blue), intraosseous chronic inflammation (ICI, orange), periosteal inflammation (PI, green), and bone necrosis (BN, yellow)