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Fig. 4 | BMC Microbiology

Fig. 4

From: The role of the globular heads of the C1q receptor in TcdA-induced human colonic epithelial cell apoptosis via a mitochondria-dependent pathway

Fig. 4

Effect of silencing of gC1qR gene on TcdA-induced cell apoptosis in human colonic epithelial cells. NCM 460 cells were transfected with gC1qR siRNA or negative siRNA for 48 h respectively, and then TcdA (10 ng/ml) was added for 24 h. a Cell viability was determined by WST-1 assay as described previously; b Apoptotic death of NCM 460 cells was examined by flow cytometric analysis. The data are means ± S.D. of three separate experiments performed in triplicate. c The expression of caspase-3 protein in lysates of NCM460 cells was measured by western blot assay. ***p < 0.001, **p < 0.01, *p < 0.05, #p > 0.05 versus TcdA (−), gC1qR siRNA (−) and negative siRNA (−) group (Mock group); p < 0.01 versus TcdA (+), gC1qR siRNA (−) and negative siRNA (−) group; ▲▲p < 0.01 versus TcdA (+), gC1qR siRNA (+) and negative siRNA (−) group. d The change of morphology of the NCM 460 cells was observed with an electron microscope. Red arrows point to the nuclei condensed and fractured, and chromatin increased and marginalized in the nucleus (3700 X)

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