Fig. 3
The effect of overexpression of gC1qR on mitochondrial function of human colonic NCM 460 epithelial cell lines. The human colonic epithelial cells were transfected with gC1qR vector or empty vector for 48 h. a Intracellular ROS generation was determined by fluorescence of H2DCFDA (Green). b The change of mitochondrial membrane potential was detected. The relative Δψm value was measured by monitoring the fluorescence of JC-1 (590: 527 nm fluorescence ratio). c ATP content was detected in NCM 460 epithelial cell. The data are means ± S.D. of three separate experiments performed in triplicate. **p < 0.01, #p > 0.05 versus Mock group