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Fig. 7 | BMC Microbiology

Fig. 7

From: Identification and characterization of the bacteriocin Carocin S3 from the multiple bacteriocin producing strain of Pectobacterium carotovorum subsp. carotovorum

Fig. 7

In vitro hydrolysis of DNA and RNA by Carocin S3. a Analysis of the DNase activity of Carocin S3. The samples are labelled as M, marker; S, genomic DNA of Ea1068; 1, genomic DNA/ddw; 2, genomic DNA/buffer (contains Mg2+); 3, genomic DNA/BamHI + buffer; 4, genomic DNA/EcoRI + buffer; 5, genomic DNA/Carocin S3I (1 μM) + buffer; 6, genomic DNA/Carocin S3K(1 μM) + buffer; 7, genomic DNA/Carocin S3K(1 μM) + Carocin S3I(1 μM) + buffer. b 0.15 μg of genomic DNA per sample were incubated with different concentration of Carocin S3I. Lane S, contains 0.15 μM genomic DNA; lane K, 0.15 μM genomic DNA and 1 μM Carocin S3K; all other lanes contain 0.15 μM genomic DNA and different concentrations of Carocin S3I protein. All samples contained 10 mM Mg2+

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