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Fig. 2 | BMC Microbiology

Fig. 2

From: Identification and characterization of the bacteriocin Carocin S3 from the multiple bacteriocin producing strain of Pectobacterium carotovorum subsp. carotovorum

Fig. 2

A Bacteriocin assays of Pectobacterium carotovorum subsp. carotovorum. Numbered strains show the wild type H-rif-8-6 (1) and the caroS1K:Tn5 insertion mutant, TH22–10 (2). Other unlabeled strains are Tn5 insertion mutants of progeny of H-rif-8-6 strain. The indicators were Pcc strain SP33 (A) and Ea1068 (B). B In vitro analysis of the DNase activity of rough protein extract from TH22–10. Samples were subjected to gel electrophoresis and labeled as follows: Lane M, the HindIII-digested λ DNA marker; 1, the equal quantities of EcoRI-digested genomic DNA; 2, the genomic DNA with protein extract from H-rif-8-6; 3, the genomic DNA with protein extract from TH22–10; 4, the genomic DNA only with reaction buffer; 5, the genomic DNA only. C Carocin S1 protein detection. Carocin S1K antiserum antibody (anti-CaroS1K) and Carocin S1I antiserum antibody (anti-CaroS1I) were detected from rabbits

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