Fig. 2From: Identification and characterization of the bacteriocin Carocin S3 from the multiple bacteriocin producing strain of Pectobacterium carotovorum subsp. carotovorumA Bacteriocin assays of Pectobacterium carotovorum subsp. carotovorum. Numbered strains show the wild type H-rif-8-6 (1) and the caroS1K:Tn5 insertion mutant, TH22–10 (2). Other unlabeled strains are Tn5 insertion mutants of progeny of H-rif-8-6 strain. The indicators were Pcc strain SP33 (A) and Ea1068 (B). B In vitro analysis of the DNase activity of rough protein extract from TH22–10. Samples were subjected to gel electrophoresis and labeled as follows: Lane M, the HindIII-digested λ DNA marker; 1, the equal quantities of EcoRI-digested genomic DNA; 2, the genomic DNA with protein extract from H-rif-8-6; 3, the genomic DNA with protein extract from TH22–10; 4, the genomic DNA only with reaction buffer; 5, the genomic DNA only. C Carocin S1 protein detection. Carocin S1K antiserum antibody (anti-CaroS1K) and Carocin S1I antiserum antibody (anti-CaroS1I) were detected from rabbitsBack to article page