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Table 2 PCR master mix 25 μl volume

From: Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay

Sr. No.

Constituent

Con.n

For

1 reaction (μl)

1

Taq buffer

10X (GeNei)

2.5

2

dNTP Mix

200 M (GeNei)

2.0

3

TaqPolymerase

5 unit

0.3

4

16S rRNA forward

10 μM

1

5

16S rRNA reverse

10 μM

1

6

MAC forward

10 μM

1

7

MAC reverse

10 μM

1

8

MK forward

10 μM

1

9

MK reverse

10 μM

1

10

MA forward

10 μM

1

11

MA reverse

10 μM

1

12

DNA

 

5

13

Deionised water

 

7.2