Sr. No. | Constituent | Con.n | For 1 reaction (μl) |
---|---|---|---|
1 | Taq buffer | 10X (GeNei) | 2.5 |
2 | dNTP Mix | 200 M (GeNei) | 2.0 |
3 | TaqPolymerase | 5 unit | 0.3 |
4 | 16S rRNA forward | 10 μM | 1 |
5 | 16S rRNA reverse | 10 μM | 1 |
6 | MAC forward | 10 μM | 1 |
7 | MAC reverse | 10 μM | 1 |
8 | MK forward | 10 μM | 1 |
9 | MK reverse | 10 μM | 1 |
10 | MA forward | 10 μM | 1 |
11 | MA reverse | 10 μM | 1 |
12 | DNA | Â | 5 |
13 | Deionised water | Â | 7.2 |