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Table 2 PCR master mix 25 μl volume

From: Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay

Sr. No. Constituent Con.n For
1 reaction (μl)
1 Taq buffer 10X (GeNei) 2.5
2 dNTP Mix 200 M (GeNei) 2.0
3 TaqPolymerase 5 unit 0.3
4 16S rRNA forward 10 μM 1
5 16S rRNA reverse 10 μM 1
6 MAC forward 10 μM 1
7 MAC reverse 10 μM 1
8 MK forward 10 μM 1
9 MK reverse 10 μM 1
10 MA forward 10 μM 1
11 MA reverse 10 μM 1
12 DNA   5
13 Deionised water   7.2