TY - JOUR AU - Chen, Xiaofeng AU - Thomsen, Trine Rolighed AU - Winkler, Heinz AU - Xu, Yijuan PY - 2020 DA - 2020/08/24 TI - Influence of biofilm growth age, media, antibiotic concentration and exposure time on Staphylococcus aureus and Pseudomonas aeruginosa biofilm removal in vitro JO - BMC Microbiology SP - 264 VL - 20 IS - 1 AB - Biofilm is known to be tolerant towards antibiotics and difficult to eradicate. Numerous studies have reported minimum biofilm eradication concentration (MBEC) values of antibiotics for many known biofilm pathogens. However, the experimental parameters applied in these studies differ considerably, and often the rationale behind the experimental design are not well described. This makes it difficult to compare the findings. To demonstrate the importance of experimental parameters, we investigated the influence of biofilm growth age, antibiotic concentration and treatment duration, and growth media on biofilm eradication. Additionally, OSTEOmycin™, a clinically used antibiotic containing allograft bone product, was tested for antibiofilm efficacy. SN - 1471-2180 UR - https://doi.org/10.1186/s12866-020-01947-9 DO - 10.1186/s12866-020-01947-9 ID - Chen2020 ER -