Skip to main content
Fig. 4 | BMC Microbiology

Fig. 4

From: Enterohaemorrhagic Escherichia coli activates nitrate respiration to benefit from the inflammatory response for initiation of microcolony-formation

Fig. 4

Effect of prestimulation of epithelial cells on EHEC adherence. HT-29 cells were exposed to heat-killed bacteria in the absence (−) or presence (+) of aminoguanidine hydrochloride 2 h prior to infection. EHEC O157 Sakai wild-type (WT) or the narGHJI mutant (ΔnarGHJI) grown under anaerobic conditions was added to the cells to initiate infection. At 3 h post-infection, cells were washed, and adherent bacteria were recovered by lysing host cells. Viable bacterial number was determined by adequate dilution and plating. The average of three independent infections along with standard error is shown. *: p < 0.05, ns: not significant

Back to article page