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Fig. 5 | BMC Microbiology

Fig. 5

From: Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis

Fig. 5

The optimal reaction time for B. pertussis-MCDA-LFB detection. Four different reaction times including 10 min (a), 20 min (b), 30 min (c), and 40 min (d) were examined and compared at 64 °C. Biosensors 1–7 indicate 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, 1 fg of DNA extracted from B. pertussis, respectively; Biosensor 8 indicates DW (blank control),. The optimal sensitivity appeared at 40 min of amplification (c). TL indicates test line; CL indicates control line

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