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Fig. 4 | BMC Microbiology

Fig. 4

From: Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis

Fig. 4

Sensitivity evaluation of B. pertussis-MCDA-LFB assay for the detection of DNA from B. pertussis strain ATCC-9340. Lateral flow biosensor (a) and colorimetric indicator (b) were used to monitor the reaction products. Biosensors (a)/Tubes (b) from 1 to 7 indicate 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, and 1 fg of target DNA; Biosensor 8 indicate DW (negative control), respectively. Amplification of 1 ng, 100 pg, 10 pg, 1 pg, 100 fg and 10 fg of DNA template showed positive amplification, while the 1 fg per reaction and blank control was negative. NC: negative control; TL: test line; CL: control line

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