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Fig. 3 | BMC Microbiology

Fig. 3

From: Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis

Fig. 3

Optimization of the reaction temperature for B. pertussis-MCDA-LFB assay. The real-time turbidity of MCDA amplification for the detection of B. pertussis was monitored, and the concentrations of DNA were shown with corresponding curves as indicated in the figures. The threshold value was 0.1 and a turbidity > 0.1 was defined as positive. Eight of kinetic graphs (1–8) were generated at different temperatures points (from 60 to 67 °C with interval of 1 °C.) with DNA template with 10 pg each reaction. Graphs 2–8 showed strong amplification

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BMC Microbiology

ISSN: 1471-2180

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