From: Intracellular Ser/Thr/Tyr phosphoproteome of the oral commensal Streptococcus gordonii DL1
Abbr | Protein | Geneb) | Acc. No.c) | pI estimated from gel/theoreticald) | MW, Da estimated from gel/ theoreticald) | LC-MS/MS | Ser/Thr/Tyr phosphory-lated +/− | ||
---|---|---|---|---|---|---|---|---|---|
MASCOT score | No. of peptidese) | Coverage | |||||||
Transmembrane transport | |||||||||
ABCa) | ABC transporter ATP-binding protein SP1715 | SGO_1342 | A8AXW3 | 6.7/6.25 | 23,000/26325 | 1124 | 18 | 72% | – |
Sugar transport | |||||||||
HPra) | Phosphocarrier protein HPr | ptsH (SGO_1556) | A8AYH5 | 5.1/4.74 | 11,000/8935 | 124 | 2 | 55% | + |
Glycolysis | |||||||||
Tpi-1a) | Triosephosphate isomerase | tpiA (SGO_0762) | A8AWA1 | 4.5/4.75 | 23,000/26524 | 652 | 9 | 52% | + |
Tpi-2a) | 4.3/4.75 | 23,000/26524 | 540 | 9 | 52% | - | |||
pfk-1a) | Phosphofructokinase | pfkA (SGO_1340) | A8AXW1 | 6.6/5.62 | 35,000/35346 | 1043 | 17 | 49% | + |
pfk-2 | 6.8/5.62 | 35,000/35346 | 1476 | 22 | 56% | + | |||
fba-1f) | Fructose-1,6-bisphosphate aldolase, class II | Fba (SGO_1745) | A8AZ06 | 5.1/5.00 | 27,000/31394 | - | - | - | - |
fba-2a) | 5.2/5.00 | 27,000/31394 | 771 | 12 | 51% | - | |||
fba-3a) | 5.3/5.00 | 27,000/31394 | 800 | 14 | 47% | - | |||
fba-4f) | 5.4/5.00 | 27,000/31394 | - | - | - | - | |||
fba-5a) | 5.4/5.00 | 17,000/31394 | 164 | 3 | 11% | - | |||
gapdh-1a) | Glyceraldehyde-3-phosphate dehydrogenase | gap (SGO_0207) | A8AUR7 | 5.5/5.37 | 39,000/35918 | 1153 | 20 | 51% | + |
gapdh-2a) | 5.7/5.37 | 39,000/35918 | 1299 | 21 | 79% | + | |||
gapdh-3a) | 5.9/5.37 | 39,000/35918 | 1304 | 22 | 58% | + | |||
gapdh-4 | 5.5/5.37 | 40,000/35918 | 89 | 2 | 8% | - | |||
gapdh-5 | 5.7/5.37 | 40,000/35918 | 276 | 4 | 14% | - | |||
gapdh-6 | 6.0/5.37 | 39,000/35918 | 84 | 2 | 6% | - | |||
gapdh-7 | 5.9/5.37 | 40,000/35918 | 325 | 5 | 16% | - | |||
gapdh-8 | 5.2/5.37 | 18,000/35918 | 141 | 2 | 8% | - | |||
pgk-1 | Phosphoglycerate kinase | pgk (SGO_0209) | A8AUR9 | 4.9/4.88 | 45,000/42089 | 397 | 6 | 18% | + |
pgk -2a) | 5.0/4.88 | 45,000/42089 | 1454 | 23 | 63% | + | |||
pgk -3a) | 4.3/4.88 | 21,000/42089 | 340 | 5 | 17% | + | |||
pgk -4f) | 5.1/4.88 | 45,000/42089 | - | - | - | - | |||
pgk -5f) | 5.2/4.88 | 45,000/42089 | - | - | - | - | |||
pgk −6 | 4.3/4.88 | 45,000/42089 | 159 | 3 | 9% | - | |||
pgk -7a) | 5.9/4.88 | 24,000/42089 | 431 | 7 | 18% | - | |||
pgk -8a) | 4.2/4.88 | 20,000/42089 | 830 | 11 | 41% | - | |||
pgk -9a) | 5.9/4.88 | 20,000/42089 | 561 | 9 | 28% | - | |||
pgm-1a) | Phosphoglycerate mutase | gpma (SGO_0704) | A8AW46 | 5.9/5.41 | 22,000/26044 | 904 | 18 | 84% | + |
pgm-2a) | 6.1/5.41 | 22,000/26044 | 933 | 18 | 87% | + | |||
eno-1 | Enolase | eno (SGO_1426) | A8AY46 | 4.4/4.71 | 48,000/47062 | 1368 | 18 | 61% | + |
eno −2 | 4.5/4.71 | 48,000/47062 | 1260 | 13 | 52% | + | |||
eno −3 | 4.6/4.71 | 48,000/47062 | 1721 | 18 | 63% | + | |||
eno -4a) | 4.7/4.71 | 48,000/47062 | 2245 | 31 | 77% | + | |||
eno −5 | 4.6/4.71 | 26,000/47062 | 1316 | 17 | 58% | + | |||
eno -6a) | 4.5/4.71 | 24,000/47062 | 460 | 8 | 26% | + | |||
eno -7a) | 4.1/4.71 | 22,000/47062 | 809 | 9 | 30% | + | |||
eno -8a) | 4.2/4.71 | 21,000/47062 | 673 | 9 | 28% | + | |||
eno -9a) | 4.3/4.71 | 21,000/47062 | 595 | 10 | 32% | + | |||
eno -10a) | 4.4/4.71 | 21,000/47062 | 989 | 11 | 34% | + | |||
eno -11a) | 4.4/4.71 | 28,000/47062 | 1485 | 15 | 55% | - | |||
eno -12a) | 4.8/4.71 | 25,000/47062 | 950 | 14 | 47% | - | |||
eno -13a) | 4.4/4.71 | 23,000/47062 | 460 | 8 | 26% | - | |||
eno -14a) | 4.2/4.71 | 23,000/47062 | 746 | 11 | 36% | - | |||
eno -15a) | 4.8/4.71 | 23,000/47062 | 627 | 9 | 32% | - | |||
eno -16a) | 4.8/4.71 | 22,000/47062 | 631 | 9 | 31% | - | |||
eno -17a) | 4.9/4.71 | 22,000/47062 | 636 | 9 | 31% | - | |||
eno -18a) | 4.4/4.71 | 17,000/47062 | 230 | 4 | 11% | - | |||
eno −19 | 4.5/4.71 | 14,500/47062 | 931 | 9 | 41% | - | |||
eno − 20 | 4.6/4.71 | 14,000/47062 | 533 | 7 | 23% | - | |||
eno -21a) | 5.0/4.71 | 13,000/47062 | 437 | 7 | 23% | - | |||
eno -22a) | 4.7/4.71 | 12,500/47062 | 722 | 9 | 33% | - | |||
eno -23a) | 4.3/4.71 | 11,000/47062 | 418 | 6 | 20% | - | |||
eno -24a) | 5.4/4.71 | 18,000/47062 | 684 | 9 | 27% | - | |||
pyk-1a) | Pyruvate kinase | pyk (SGO_1339) | A8AXW0 | 5.15/4.94 | 58,000/54799 | 150 | 3 | 6% | + |
pyk-2a) | 5.2/4.94 | 58,000/54799 | 1474 | 29 | 62% | + | |||
pyk-3f) | 5.1/4.94 | 58,000/54799 | - | - | - | - | |||
pyk-4f) | 5.3/4.94 | 58,000/54799 | - | - | - | - | |||
Cofactor biosynthesis | |||||||||
nadea) | NH(3)-dependent NAD(+) synthetase | nadE (SGO_0583) | A8AVT9 | 5.4/5.11 | 35,000/30248 | 268 | 3 | 16% | – |
Acetoin catabolism | |||||||||
aced-1 | Acetoin dehydrogenase | butA (SGO_1096) | A8AX75 | 5.4/5.20 | 26,000/26547 | 1008 | 15 | 77% | - |
aced-2a) | 5.7/5.20 | 26,000/26547 | 1247 | 17 | 75% | - | |||
aced-3a) | 5.7/5.20 | 24,000/26547 | 809 | 13 | 62% | - | |||
Carbohydrate catabolism | |||||||||
deraa) | Deoxyribose-phosphate aldolase | deoC (SGO_1080) | A8AX59 | 4.9/4.84 | 20,000/23380 | 691 | 8 | 49% | – |
Pyruvate conversion | |||||||||
ldh-1a) | L-lactate dehydrogenase | ldh (SGO_1232) | A8AXK9 | 5.4/5.24 | 36,000/35267 | 327 | 6 | 28% | + |
ldh-2 | 5.6/5.24 | 36,000/35267 | 436 | 6 | 28% | + | |||
ldh-3 | 5.8/5.24 | 36,000/35267 | 641 | 8 | 32% | - | |||
adha) | Alcohol dehydrogenase | adhA (SGO_0565) | A8AVS1 | 5.2/4.94 | 39,000/35958 | 911 | 13 | 69% | – |
pox | Pyruvate oxidase | spxB (SGO_0292) | A8AV01 | 5.3/5.06 | 65,000/65283 | 161 | 3 | 5% | – |
Pentose phosphate pathway | |||||||||
deob | Phosphopentomutase | deoB (SGO_1264) | A8AXN8 | 5.3/4.96 | 47,000/44508 | 172 | 3 | 9% | + |
Amino acid metabolism | |||||||||
alad | Alanine dehydrogenase | ald (SGO_0708) | A8AW50 | 6.1/5.29 | 45,000/38960 | 1378 | 19 | 63% | – |
dapha) | 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase | dapH (SGO_0158) | A8AUL9 | 5.2/5.06 | 22,000/24255 | 291 | 5 | 25% | – |
glya | Serine hydroxymethyltransferase | glyA, (SGO_1151) | A8AXC8 | 5.4/5.07 | 48,000/45566 | 583 | 8 | 28% | – |
sed | Homoserine dehydrogenase | hom (SGO_0801) | A8AWE0 | 5.0/4.86 | 45,000/46154 | 245 | 3 | 11% | – |
Nucleotide metabolism | |||||||||
adka) | Adenylate kinase | adk (SGO_1964) | A8AZK4 | 5.2/4.95 | 22,000/ 23,857 | 318 | 6 | 38% | – |
aprta) | Adenine phosphoribosyltransferase | apt (SGO_1001) | A8AWY0 | 5.0/4.90 | 17,000/18840 | 875 | 12 | 74% | – |
imdh-1 | Inosine-5′-monophosphate dehydrogenase | guaB (SGO_0008) | A8AU70 | 5.4/5.19 | 58,000/52717 | 116 | 2 | 5% | + |
imdh-2 | 5.5/5.19 | 56,000/52717 | 252 | 4 | 12% | - | |||
pyrb | Aspartate carbamoyltransferase | pyrB (SGO_1109) | A8AX88 | 5.6/5.04 | 35,000/34741 | 54 | 1g) | 4% | – |
xprt | Xanthine phosphoribosyltransferase | xpt (SGO_1158) | A8AXD5 | 6.2/5.79 | 17,000/20849 | 684 | 12 | 77% | – |
Molecular chaperone activity and/or protein folding | |||||||||
Dnak-1 | Molecular chaperone DnaK | dnaK (SGO_0402) | A8AVA8 | 4.4/4.69 | 68,000/64724 | 266 | 4 | 9% | + |
DnaK-2a) | 4.5/4.69 | 68,000/64724 | 1557 | 26 | 50% | + | |||
DnaK-3a) | 5.2/4.69 | 25,000/64724 | 134 | 2 | 3% | + | |||
DnaK-4f) | 4.6/4.69 | 68,000/64724 | - | - | - | - | |||
DnaK-5 | 4.1/4.69 | 44,000/64724 | 254 | 4 | 10% | - | |||
DnaK-6a) | 5.3/4.69 | 36,000/64724 | 345 | 6 | 14% | - | |||
GroEL-1 | Molecular chaperone GroEL | groEL (SGO_1885) | A8AZE1 | 4.2/4.66 | 64,000/56754 | 776 | 13 | 34% | + |
GroEL-2 | 4.3/4.66 | 64,000/56754 | 1500 | 22 | 47% | + | |||
GroEL-3 | 4.4/4.66 | 64,000/56754 | 794 | 13 | 32% | - | |||
GroES | Co-chaperone GroES | groES (SGO_1886) | A8AZE2 | 5.4/5.11 | 64,000/9681 | 65 | 1g) | 2% | – |
grpEa) | Molecular chaperone GrpE | grpE (SGO_0401) | A8AVA7 | 4.8/4.83 | 23,000/20265 | 186 | 3 | 18% | – |
hyda) | Hydrolase, haloacid dehalogenase family/ peptidyl-prolyl cis-trans isomerase, cyclophilin type | SGO_0604 | A8AVW0 | 4.2/4.74 | 22,000/51936 | 516 | 10 | 30% | – |
tiga) | Trigger factor (chaperone) | tig (SGO_0412) | A8AVB8 | 4.2/4.53 | 55,000/47242 | 531 | 10 | 31% | + |
Transcription | |||||||||
GreA | Transcription elongation factor GreA | greA (SGO_0519) | A8AVM5 | 4.9/4.85 | 15,500/17515 | 581 | 9 | 68% | – |
rpoAa) | DNA-directed RNA polymerase subunit alpha | rpoA (SGO_1959) | A8AZJ9 | 4.5/4.70 | 41,000/34502 | 578 | 7 | 29% | – |
rpoZ | DNA-directed RNA polymerase subunit omega | rpoZ (SGO_0595) | A8AVV1 | 6.7/5.82 | 12,000/11851 | 231 | 5 | 44% | – |
y454 | Probable transcriptional regulatory protein | A8AVG0 | 4.6/4.45 | 30,000/25722 | 227 | 4 | 26% | – | |
Translation | |||||||||
ef-G | Elongation factor G | fusA (SGO_0206) | A8AUR6 | 4.7/4.88 | 50,000/76785 | 53 | 1g) | 2% | – |
ef-Ts | Elongation factor Ts | tsf (SGO_2000) | A8AZP0 | 4.9/4.84 | 41,000/37284 | 326 | 5 | 20% | – |
ef-tu-1a) | Elongation factor tu | tuf (SGO_0761) | A8AWA0 | 4.1/4.86 | 55,000/44011 | 519 | 8 | 31% | + |
ef-tu-2 | 5–6/4.86 | 55,000/44011 | 1253 | 22 | 63% | + | |||
ef-tu-3a) | 4.2/4.86 | 34,000/44011 | 532 | 6 | 23% | + | |||
ef-tu-4a) | 4.3/4.86 | 25,000/44011 | 167 | 3 | 8% | + | |||
ef-tu-5a) | 4.3/4.86 | 29,000/44011 | 553 | 6 | 25% | - | |||
ef-tu-6a) | 4.7/4.86 | 23,000/44011 | 420 | 6 | 18% | - | |||
ef-tu-7a) | 5.3/4.86 | 20,000/44011 | 433 | 6 | 16% | - | |||
rL1a) | 50S ribosomal protein L1 | rplA (SGO_1455) | A8AY74 | 5.5/9.22 | 23,000/24399 | 202 | 3 | 16% | – |
rL4-1a) | 50S ribosomal protein L4 | rplD (SGO_1984) | A8AZM4 | 5.2/10.03 | 19,000/22279 | 285 | 4 | 26% | - |
rL4-2a) | 5.5/10.03 | 19,000/22279 | 327 | 4 | 24% | - | |||
rL10a) | 50S ribosomal protein L10 | rplJ (SGO_1192) | A8AXG9 | 5.2/5.06 | 15,000/17462 | 1002 | 15 | 86% | – |
rL17 | 50S ribosomal protein L17 | rplQ (SGO_1958) | A8AZJ8 | 5.3/9.86 | 13,000/14511 | 141 | 2 | 17% | – |
rrfa) | Ribosome recycling factor | frr (SGO_1451) | A8AY70 | 6.8/5.90 | 18,000/20640 | 421 | 8 | 46% | – |
rS1 | 30S ribosomal protein S1 | rpsA (SGO_1234) | A8AXL0 | 4.5/4.91 | 52,000/44149 | 130 | 2 | 5% | + |
rS2 | 30S ribosomal protein S2 | rpsB (SGO_2001) | A8AZP1 | 4.6/5.07 | 33,000/29125 | 515 | 8 | 35% | – |
rS5-1a) | 30S ribosomal protein S5 | rpsE (SGO_1968) | A8AZK8 | 5.1/9.69 | 14,000/17079 | 335 | 4 | 33% | - |
rS5–2 | 5.3/9.69 | 14,000/17079 | 283 | 4 | 33% | - | |||
rS8a) | 30S ribosomal protein S8 | rpsH (SGO_1971) | A8AZL1 | 5.3/9.48 | 12,500/14753 | 120 | 2 | 25% | – |
rS17 | 30S ribosomal protein S17 | rpsQ (SGO_1976) | A8AZL6 | 5.0/9.92 | 11,000/10010 | 58 | 1g) | 10% | – |
sys-1 | Serine tRNA-ligase | serS (SGO_1683) | A8AYV0 | 5.4/5.11 | 48,000/48110 | 342 | 7 | 21% | - |
sys-2a) | 5.7/5.11 | 21,000/48110 | 95 | 1g) | 2% | - | |||
Protein catabolism | |||||||||
clpp | ATP-dependent Clp protease proteolytic subunit | clpP (SGO_1632) | A8AYP9 | 4.3/4.72 | 18,000/21363 | 200 | 3 | 12% | – |
pept | Peptidase T | pepT (SGO_1312) | A8AXT3 | 4.2/4.59 | 49,000/45200 | 332 | 4 | 13% | – |
Vitamin B biosynthesis | |||||||||
pxka) | Pyridoxine kinase | SGO_0409 | A8AVB5 | 5.3/5.16 | 23,000/27598 | 583 | 10 | 52% | – |
Cell envelope biosynthesis | |||||||||
murda) | UDP-N-acetylmuramoylalanine D-glutamate ligase | murdD (SGO_0671) | A8AW14 | 5.4/5.07 | 46,000/48257 | 253 | 4 | 12% | – |
pgama) | Phosphoglucosamine mutase | glmM (SGO_0889) | A8AWM5 | 4.6/4.71 | 52,000/48396 | 592 | 7 | 26% | + |
rmla-1 | Glucose-1-phosphate thymidylyltransferase | rfbA-1 (SGO_1009) | A8AWY8 | 5.1/4.92 | 28,000/32213 | 42 | 1g) | 3% | + |
rmla-2a) | 5.3/4.92 | 28,000/32213 | 136 | 2 | 7% | + | |||
rmlb | dTDP-glucose 4,6-dehydratase | rfbB-1 (SGO_1011) | A8AWZ0 | 6.3/5.55 | 40,000/39314 | 168 | 3 | 12% | – |
Cell division | |||||||||
SepF | Cell division protein SepF | sepF (SGO_0677) | A8AW20 | 6.6/5.61 | 19,000/21654 | 367 | 6 | 41% | – |
Antioxidant activity | |||||||||
soda) | Superoxide dismutase | sodA (SGO_1599) | A8AYL7 | 4.9/4.78 | 19,000/22446 | 474 | 8 | 46% | – |
tox | Thiol peroxidase | Tpx (SGO_1803) | A8AZ62 | 4.2/4.52 | 16,000/18015 | 349 | 6 | 59% | – |
Protein dephosphorylation | |||||||||
pppa) | Phosphoprotein phosphatase SGO_0599 | SGO_0599 | A8AVV5 | 4.3/4.62 | 23,000/26870 | 696 | 11 | 61% | + |