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Table 1 Strains and plasmids used in this study

From: The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatis

Strain or plasmid Relevant characteristic Source or reference
M. smegmatis mc2155 WT, ATCC19420 [29]
aceE-mut mc2155 with aceE-mut disrupted by Himar1 transposon This study
 Vector aceE-mut complemented with pSMT3-M plasmid This study
 Comp aceE-mut complemented with pSMT3-aceE This study
 Mar T7   [28]
 pSMT3 Carries hygr, E. coli-mycobacterial shuttle vector [30]
 pSMT3-aceE aceE gene cloned under hsp60 promoter in pSMT3-M vector This study