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Table 1 Strains and plasmids used in this study

From: The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatis

Strain or plasmid

Relevant characteristic

Source or reference

Strains

 M. smegmatis mc2155

WT, ATCC19420

[29]

 aceE-mut

mc2155 with aceE-mut disrupted by Himar1 transposon

This study

 Vector

aceE-mut complemented with pSMT3-M plasmid

This study

 Comp

aceE-mut complemented with pSMT3-aceE

This study

Plasmids

 Mar T7

 

[28]

 pSMT3

Carries hygr, E. coli-mycobacterial shuttle vector

[30]

 pSMT3-aceE

aceE gene cloned under hsp60 promoter in pSMT3-M vector

This study