From: The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatis
Strain or plasmid | Relevant characteristic | Source or reference |
---|---|---|
Strains | ||
 M. smegmatis mc2155 | WT, ATCC19420 | [29] |
 aceE-mut | mc2155 with aceE-mut disrupted by Himar1 transposon | This study |
 Vector | aceE-mut complemented with pSMT3-M plasmid | This study |
 Comp | aceE-mut complemented with pSMT3-aceE | This study |
Plasmids | ||
 Mar T7 |  | [28] |
 pSMT3 | Carries hygr, E. coli-mycobacterial shuttle vector | [30] |
 pSMT3-aceE | aceE gene cloned under hsp60 promoter in pSMT3-M vector | This study |