Fig. 2From: The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatisThe aceE-mut strain was sensitive to acid stress. a and b. Bacterial strains were grown in the neutral Middlebrook 7H9 medium supplemented with 0.05% Tween-80 and 0.2% glycerol; c and d. Bacterial strains were grown in the acified Middlebrook 7H9 medium (pH 5.0) supplemented with 0.05% Tween-80 and 0.2% glycerol. The OD600 and CFU were determined at an interval of 24 h. The graph is a representation of one of three independent experiments. The mean ± SD of triplicate experimental samples is shown from one experiment, Error bars represent SDBack to article page