Fig. 1
From: The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatis
The identification of M. smegmatis aceE-mut. a. The aceE-mut showed smoother colony morphology in contrast to WT. The culture of M. smegmatis mc2155 and aceE-mut were 10× diluted and 10 μL culture aliquots were spotted on the Middlebrook 7H10 medium supplemented with 0.2% glycerol. The images were taken after incubation at 37 °C for 3 days on 7H10 plates. b. HimarI transposon insertion site in aceE gene; c. PCR verification of the aceE transposon mutant. WT: M. smegmatis mc2155; aceE-mut: aceE gene deficiency mutant selected from M. smegmatis mc2155 transposon library