Fig. 3

Construction of pCSN44-∆FocSge1 deletion vector (a) and pBC-Phleo ∆FocSge1C/T complementation or truncated vector (b). Screening of the transformants using FocSge1 gene coding sequence primers (c) and hygromycin resistant gene coding sequence primers (d). Quantitative real-time RT-PCR showed differential expression of FocSge1 gene in wild-type, complementation mutant C7 strain and truncated mutant T10 strain in presence of banana cells whereas no expression was detected in deletion mutant exposed to banana cells (e). Dunnett’s multiple comparisons test was performed to determine the significance between the expression levels of wild-type and mutant strains (**** P < 0.0001, * P < 0.05, ns- non-significant)