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Table 1 The gene information of GPI proteins in deletion library strains

From: Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris

Strains

Deletion gene

Gene name

Size (AA)

NCBI BLAST

Domain description

gcw1Δ

GCW1

538

β-1,3-Glucanosyltransferase

Glucanosyltransferase; X8; Glycoside hydrolase

gcw2Δ

GCW2

535

β-1,3-Glucanosyltransferase

Glucanosyltransferase; X8; Glycoside hydrolase

ku70Δ gcw3Δ

GCW3

307

Hypothetical protein

Superoxide dismutase, Cu/Zn binding domain

gcw4Δ

GCW4

266

Hypothetical protein

No hits found

gcw5Δ

GCW5

203

Hypothetical protein

Flocculin type 3 repeat

gcw6Δ

GCW6

448

Cell wall protein

Glycoside hydrolase, predicted CRH1;Concanavalin A-like lectin/glucanases

gcw7Δ

GCW7

593

Aspartic protease

Peptidase A1; Aspartic peptidase

ku70Δ gcw8Δ

GCW8

448

Putative mannosidase

Glycoside hydrolase; Six-hairpin glycosidase; Mannanendo-1,6-alpha-mannosidase

gcw10Δ

GCW10

251

Hypothetical protein

No hits found

gcw12Δ

GCW12

233

CFEM protein

Extracellular membrane protein, CFEM domain

gcw13Δ

GCW13

294

Hypothetical protein

No hits found

gcw14Δ

GCW14

135

Hypothetical protein

No hits found

gcw15Δ

GCW15

237

Hypothetical protein

No hits found

gcw16Δ

GCW16

1416

Mucin-like protein

Cellulose-binding domain; Uncharacterized domain Flo11-related

gcw17Δ

GCW17

259

Hypothetical protein

No hits found

–

GCW18

1667

Hypothetical protein

Peptidase S8/S53 domain; PT repeat, subtilisin-related

gcw19Δ

GCW19

148

Hypothetical protein

No hits found

gcw21Δ

GCW21

233

Hypothetical protein

No hits found

gcw22Δ

GCW22

839

Hypothetical protein

Uncharacterized domain Flo11-related, N-terminal; Flocculin type 3 repeat

–

GCW23

418

Cell wall protein

Receptor L domain-like

ku70Δ gcw24Δ

GCW24

261

Hypothetical protein

No hits found

ku70Δ gcw25Δ

GCW25

595

Hypothetical protein

Flocculin type 3 repeat

ku70Δ gcw26Δ

GCW26

724

Hypothetical protein

GLEYA adhesin domain

ku70Δ gcw28Δ

GCW28

587

Mucin-like protein

Adhesion domain, bacterial

–

GCW29

1474

Hypothetical protein

PT repeat; Adhesion domain, bacterial

ku70Δ gcw30Δ

GCW30

229

Hypothetical protein

No hits found

ku70Δ gcw31Δ

GCW31

562

Hypothetical protein

Peptidase A1; Aspartic peptidase

ku70Δ gcw32Δ

GCW32

216

Hypothetical protein

No hits found

–

GCW33

527

Aspartic protease

Peptidase A1; Aspartic peptidase

ku70Δ gcw34Δ

GCW34

473

Aspartic protease

Peptidase A1; Aspartic peptidase

ku70Δ gcw35Δ

GCW35

582

Putative aspartic protease

Peptidase A1; Aspartic peptidase

ku70Δ gcw36Δ

GCW36

468

Hypothetical protein

PT repeat

ku70Δ gcw37Δ

GCW37

234

Hypothetical protein

No hits found

ku70Δ gcw39Δ

GCW39

612

Aspartic protease

Peptidase A1; Aspartic peptidase

ku70Δ gcw42Δ

GCW42

248

Hypothetical protein

No hits found

ku70Δ gcw43Δ

GCW43

443

Hypothetical protein

EGF receptor, L domain

ku70Δ gcw45Δ

GCW45

409

Hypothetical protein

PT repeat; Flocculin type 3 repeat

ku70Δ gcw46Δ

GCW46

633

Phospholipase B

Lysophospholipase, catalytic domain; Acyl transferase/acyl hydrolase

ku70Δ gcw48Δ

GCW48

369

Putative protease

SUN Family

ku70Δ gcw49Δ

GCW49

327

Hypothetical protein

No hits found

–

GCW50

454

Hypothetical protein

No hits found

ku70Δ gcw51Δ

GCW51

211

Hypothetical protein

No hits found

ku70Δ gcw52Δ

GCW52

365

Hypothetical protein

No hits found

ku70Δ gcw53Δ

GCW53

354

Hypothetical protein

No hits found

ku70Δ gcw54Δ

GCW54

611

Hypothetical protein

No hits found

ku70Δ gcw56Δ

GCW56

400

Hypothetical protein

No hits found

ku70Δ gcw58Δ

GCW58

194

Hypothetical protein

Stress-induced protein SRP1/TIP1

ku70Δ gcw59Δ

GCW59

469

Putative chitin transglycosidase

Glycoside hydrolase, predicted CRH1;Concanavalin A-like lectin/glucanases

ku70Δ gcw60Δ

GCW60

599

Aspartic protease

Peptidase A1; Aspartic peptidase

gcw61Δ

GCW61

65

Hypothetical protein

No hits found