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Table 1 The gene information of GPI proteins in deletion library strains

From: Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris

Strains Deletion gene
Gene name Size (AA) NCBI BLAST Domain description
gcw1Δ GCW1 538 β-1,3-Glucanosyltransferase Glucanosyltransferase; X8; Glycoside hydrolase
gcw2Δ GCW2 535 β-1,3-Glucanosyltransferase Glucanosyltransferase; X8; Glycoside hydrolase
ku70Δ gcw3Δ GCW3 307 Hypothetical protein Superoxide dismutase, Cu/Zn binding domain
gcw4Δ GCW4 266 Hypothetical protein No hits found
gcw5Δ GCW5 203 Hypothetical protein Flocculin type 3 repeat
gcw6Δ GCW6 448 Cell wall protein Glycoside hydrolase, predicted CRH1;Concanavalin A-like lectin/glucanases
gcw7Δ GCW7 593 Aspartic protease Peptidase A1; Aspartic peptidase
ku70Δ gcw8Δ GCW8 448 Putative mannosidase Glycoside hydrolase; Six-hairpin glycosidase; Mannanendo-1,6-alpha-mannosidase
gcw10Δ GCW10 251 Hypothetical protein No hits found
gcw12Δ GCW12 233 CFEM protein Extracellular membrane protein, CFEM domain
gcw13Δ GCW13 294 Hypothetical protein No hits found
gcw14Δ GCW14 135 Hypothetical protein No hits found
gcw15Δ GCW15 237 Hypothetical protein No hits found
gcw16Δ GCW16 1416 Mucin-like protein Cellulose-binding domain; Uncharacterized domain Flo11-related
gcw17Δ GCW17 259 Hypothetical protein No hits found
GCW18 1667 Hypothetical protein Peptidase S8/S53 domain; PT repeat, subtilisin-related
gcw19Δ GCW19 148 Hypothetical protein No hits found
gcw21Δ GCW21 233 Hypothetical protein No hits found
gcw22Δ GCW22 839 Hypothetical protein Uncharacterized domain Flo11-related, N-terminal; Flocculin type 3 repeat
GCW23 418 Cell wall protein Receptor L domain-like
ku70Δ gcw24Δ GCW24 261 Hypothetical protein No hits found
ku70Δ gcw25Δ GCW25 595 Hypothetical protein Flocculin type 3 repeat
ku70Δ gcw26Δ GCW26 724 Hypothetical protein GLEYA adhesin domain
ku70Δ gcw28Δ GCW28 587 Mucin-like protein Adhesion domain, bacterial
GCW29 1474 Hypothetical protein PT repeat; Adhesion domain, bacterial
ku70Δ gcw30Δ GCW30 229 Hypothetical protein No hits found
ku70Δ gcw31Δ GCW31 562 Hypothetical protein Peptidase A1; Aspartic peptidase
ku70Δ gcw32Δ GCW32 216 Hypothetical protein No hits found
GCW33 527 Aspartic protease Peptidase A1; Aspartic peptidase
ku70Δ gcw34Δ GCW34 473 Aspartic protease Peptidase A1; Aspartic peptidase
ku70Δ gcw35Δ GCW35 582 Putative aspartic protease Peptidase A1; Aspartic peptidase
ku70Δ gcw36Δ GCW36 468 Hypothetical protein PT repeat
ku70Δ gcw37Δ GCW37 234 Hypothetical protein No hits found
ku70Δ gcw39Δ GCW39 612 Aspartic protease Peptidase A1; Aspartic peptidase
ku70Δ gcw42Δ GCW42 248 Hypothetical protein No hits found
ku70Δ gcw43Δ GCW43 443 Hypothetical protein EGF receptor, L domain
ku70Δ gcw45Δ GCW45 409 Hypothetical protein PT repeat; Flocculin type 3 repeat
ku70Δ gcw46Δ GCW46 633 Phospholipase B Lysophospholipase, catalytic domain; Acyl transferase/acyl hydrolase
ku70Δ gcw48Δ GCW48 369 Putative protease SUN Family
ku70Δ gcw49Δ GCW49 327 Hypothetical protein No hits found
GCW50 454 Hypothetical protein No hits found
ku70Δ gcw51Δ GCW51 211 Hypothetical protein No hits found
ku70Δ gcw52Δ GCW52 365 Hypothetical protein No hits found
ku70Δ gcw53Δ GCW53 354 Hypothetical protein No hits found
ku70Δ gcw54Δ GCW54 611 Hypothetical protein No hits found
ku70Δ gcw56Δ GCW56 400 Hypothetical protein No hits found
ku70Δ gcw58Δ GCW58 194 Hypothetical protein Stress-induced protein SRP1/TIP1
ku70Δ gcw59Δ GCW59 469 Putative chitin transglycosidase Glycoside hydrolase, predicted CRH1;Concanavalin A-like lectin/glucanases
ku70Δ gcw60Δ GCW60 599 Aspartic protease Peptidase A1; Aspartic peptidase
gcw61Δ GCW61 65 Hypothetical protein No hits found