Fig. 3From: Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastorisGrowth characteristics and hydrolytic activity of recombinant strains. Recombinant Cells were induced to express CALB in BMMY medium containing 1% (v/v) methanol for 120 h. The hydrolytic activity was measured using pNPB as the substrate. a and c, Growth curves of deletion strains. b and d, CALB hydrolytic activity curves of deletion strains. The control strains were GS115/CALB-GCW61 and ku70Δ/CALB-GCW61Back to article page