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Fig. 1 | BMC Microbiology

Fig. 1

From: Scanning electron microscopic observation of the in vitro cultured protozoan, Perkinsus olseni, isolated from the Manila clam, Ruditapes philippinarum

Fig. 1

External structures of P. olseni during zoosporulation, observed under light microscopy (a and c) and FE-SEM (b, d, e, f, g, h, i, and j). a. Prezoosporangia on the second day in seawater taken under a light microscope; scale bar = 50 μm. b. Prezoosporangia on the second day in seawater taken under SEM; scale bar = 25 μm. c. Zoosporangia on the third day and a 4-cell stage of zoosporangium with the discharge tube (arrow); scale bar = 50 μm. d. Initiation of discharge tube formation (arrow) on the third day; scale bar = 25 μm. e. Development of the discharge tube (arrow) on the third day; scale bar = 20 μm. f. Higher magnification of the discharge tube showing the closed membrane mesh-like structure; scale bar = 5 μm. g. Fully developed discharge tube on the fourth day; scale bar = 15 μm. h. Magnified view of the discharge tube; the tube looks like a porous sponge; scale bar = 5 μm. i. Disintegrated discharge tube and release of zoospores from the zoosporangium on the fifth day; scale bar = 10 μm. j. Empty zoosporangium after complete discharge; scale bar = 10 μm

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BMC Microbiology

ISSN: 1471-2180

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