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Fig. 8 | BMC Microbiology

Fig. 8

From: Identification and characteristics of a novel cecropin from the armyworm, Mythimna separata

Fig. 8

Expression and purification of recombinant AC-1 in E. coli. a: Expression and solubility of recombinant AC-1 in E. coli. Lane M, molecular weight marker; lane 1, E. colibl21 (DE3)/pET-32a (+); supernatant (lane 2) and precipitate (lane 3) from E. colibl21 (DE3)/pET-32a (+)-AC-1 induced at 20 °C; supernatant (lane 4) and precipitate (lane 5) from E. colibl21 (DE3)/pET-32a (+)-AC-1 induced at 37 °C. b: Optimization of purification conditions for recombinant AC-1. Lane M, molecular weight marker; lane 1, treated inclusion body lysate; lane 2, flowthrough through an Ni-NTA gravity column; lane 3, solution eluted using 20 mmol/L imidazole eluent; lane 4, solution eluted using 50 mM imidazole eluent; lane 5, solution eluted using 200 mmol/L imidazole eluent. c: Purification of recombinant AC-1. Lane M, molecular weight marker; lane 1, purified recombinant protein AC-1 through Ni-NTA gravity column. d: Tricine-SDS-PAGE analysis of AC-1. Lane M, molecular weight marker; lane 1 and 2, purified AC-1 after recombinant protein AC-1 was digested by enterokinase

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