Genetic diversity of aflatoxin-producing Aspergillus flavus isolated from selected groundnut growing agro-ecological zones of Uganda

Table 5 Primer pairs that were used in the amplification of the aflatoxin biosynthesis gene [40]

Marker	Forward 5′ → 3’	Reverse 5′ → 3’	Amplicon size (bp)
AFLC01	CCGACCTCACGACGCATTAT	CCGGCTAGCTTCAACAGACG	140–370
AFLC02	GGTTGGCGGATTGAGAGGTA	GGAGATCAGCCGAGAAGACA	100–296
AFLC03	TCCGCCGAGAGCCATAATAG	GGATGCTGACACCTCGATAG	120–160
AFLC07	GTCAGCAAGAGGAGCCTTCA	GGTCACGGAGATCCTCCATA	159–404
AFLC08	CGCCAGCACGGAGATCGAAT	CGTCTCCTCAGGCGGTCTAT	224–399
AFLC12	CGCAAGGAGCTCGACCAATA	TTCAGCTCAGCGACGAGAGT	241–360
AFLC13	TCGGTTCAATGCTCGAACAC	TCCAACCTTCGGCCTAGTCT	140–410
AFLC15	GCTCTACAGGCTGATTCAAG	TCGACAGTCCGACAATATGC	204–370
AFLC16	ATCGCAGCGGAAGCTTGGAA	AGTCTCGGACTCCGGTGACA	145–410
AFLC17	GCACAACTCGTACAGCTATC	TCTAAGTGCGAGGCAACGAA	125–390
AFLC18	GGCAGCCAGACCAAGGAATA	CCTTCTCGTAGCCGCTCATC	130–400
AFLC19	ACAGGACCGCACGGATCAAT	AGGAGCGGATGTCGAAGTCT	260–491
AFLC20	GCCTAGCGCTCCATTCTCAG	CCATCGTATCCGGCTCTATC	120–370
AFLC21	TACCTTACTCCGCTAAGCAG	GCGGTCACCTACCAATGAAT	150–368
AFLC22	TTCGCAGGAGTGTAGCCAAG	GTTGGAACACGCTCCATAGG	120–371
AFLC24	GAACGAGATAACGGCTGCAT	ATCAATCCACGGACCGTTGT	100–430

ISSN: 1471-2180