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Table 2 Primers used in this study

From: Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation

Primer name

Primer sequence (5′ to 3′)

Target

AB1

GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC

For transposon insertion sequencing

AB2

GGCCACGCGTCGACTAGTACNNNNNNNNNNCCTGG

For transposon insertion sequencing

AB3

GGCCACGCGTCGACTAGTACNNNNNNNNNNCCTCG

For transposon insertion sequencing

ABS

GGCCACGCGTCGACTAGTAC

For transposon insertion sequencing

SP1

GCTGACCGCTTCCTCGTGCTTTACG

For transposon insertion sequencing

SP2

CATCGCCTTCTATCGCCTTCTTGAC

For transposon insertion sequencing

pSC189-seq

CGCGAAGTTCCTATTCCGAAGTTCC

For transposon insertion sequencing

pDM4-F

GGTGCTCCAGTGGCTTCTGTTTCTA

For deletion mutants

pDM4-R

CAGCAACTTAAATAGCCTCTAAT

For deletion mutants

dam-up-F

GAGCGGATAACAATTTGTGGAATCCCGGGAGACGCCGAAAGCGACCACCACGACG

For dam deletion mutant

dam-up-R

TTGAGAATTACATGCTGACTAACTAATTACACCTT

For dam deletion mutant

dam-down-F

AGTCAGCATGTAATTCTCAAGGAGAAGCGGATGAA

For dam deletion mutant

dam-down-R

AGCGGAGTGTATATCAAGCTTATCGATACCCGATCTCGCCGATATTGTTCACCTT

For dam deletion mutant

dam-in-F

CGAGTGCCTTGTCGAACCTTTTGTG

For dam deletion mutant

dam-in-R

ACAGAGCCAGCAGTTCGTCCACCTT

For dam deletion mutant

dam-out-F

AACCACGACTGCGGAACCGAAGAAA

For dam deletion mutant

dam-out-R

TCGGGTTTATCGAAAATTGCCGACC

For dam deletion mutant

invC-up-F

GAGCGGATAACAATTTGTGGAATCCCGGGAGTCCTCCTTACGTCTGTCGATGTCC

For invC deletion mutant

invC-up-R

GCGAATGCATTCATCTCATTAGCGACCGACTAAAA

For invC deletion mutant

invC-down-F

AATGAGATGAATGCATTCGCTGACCAGAATTAAAG

For invC deletion mutant

invC-down-R

AGCGGAGTGTATATCAAGCTTATCGATACCAATGCTTCTGATAAACCGCCAACCT

For invC deletion mutant

invC-in-F

GGGAACGCACCGTGTTGAGCCTTAT

For invC deletion mutant

invC-in-R

CCAGGACGATATTCTCCCAAGTCAA

For invC deletion mutant

invC-out-F

CAGTACCTTCCTCAGCCTTGACCCG

For invC deletion mutant

invC-out-R

GCATTACGAAAGCATCGCCATAGTC

For invC deletion mutant

hilD-up-F

GAGCGGATAACAATTTGTGGAATCCCGGGAGCTCATGGAGTATAATTTCGGTCGT

For hilD deletion mutant

hilD-up-R

AAAAATGTTACATATTATCCCTTTGTTGATGTTAT

For hilD deletion mutant

hilD-down-F

GGATAATATGTAACATTTTTTGTATCTGTCACTTA

For hilD deletion mutant

hilD-down-R

AGCGGAGTGTATATCAAGCTTATCGATACCTGAAAGACTGTTTTTAATGGTGCGC

For hilD deletion mutant

hilD-in-F

GTCAGACTCAGCAGGTTACCATCAA

For hilD deletion mutant

hilD-in-R

CATTATGGTTGCCTATGCGTAAAAG

For hilD deletion mutant

hilD-out-F

TTCACCGACCTGTATTGGCGTATTT

For hilD deletion mutant

hilD-out-R

TTTTGGGGTGTAAATGCTGCTTATT

For hilD deletion mutant

prgH-up-F

GAGCGGATAACAATTTGTGGAATCCCGGGACACCAACATCCCAGGTTCGTCACAG

For prgH deletion mutant

prgH-up-R

CGTTAAATTACATATATACTGTTAGCGATGTCTGT

For prgH deletion mutant

prgH-down-F

AGTATATATGTAATTTAACGTAAATAAGGAAGTCA

For prgH deletion mutant

prgH-down-R

AGCGGAGTGTATATCAAGCTTATCGATACCTTCAACAGCCCCGACTCCTTTACGA

For prgH deletion mutant

prgH-in-F

GTTTGCTGCTCGTTTGGGATAAGTG

For prgH deletion mutant

prgH-in-R

GGCAAGGGTCATTACCAGCAGAAAG

For prgH deletion mutant

prgH-out-F

GAACGGCTGTGAGTTTCCATTGCTG

For prgH deletion mutant

prgH-out-R

GACGGGCTCTGAGTATTTCTACATC

For prgH deletion mutant

spaN-up-F

GAGCGGATAACAATTTGTGGAATCCCGGGAATTGACTTGGGAGAATATCGTCCTG

For spaN deletion mutant

spaN-up-R

AATGACATCACATTAAATTATCTCCTCTGACTCGG

For spaN deletion mutant

spaN-down-F

TAATTTAATGTGATGTCATTGCGTGTGAGACAGAT

For spaN deletion mutant

spaN-down-R

AGCGGAGTGTATATCAAGCTTATCGATACCCGCACGGGAAGTACGAATCAGGAGT

For spaN deletion mutant

spaN-in-F

CGACTATGGCGATGCTTTCGTAATG

For spaN deletion mutant

spaN-in-R

CAAACGATGTTCAACCTGCGTATTT

For spaN deletion mutant

spaN-out-F

AAAGCGTAAGCCGCGTTTTTGGACA

For spaN deletion mutant

spaN-out-R

CAATTGATTCAAGCCAGGCAGAGTT

For spaN deletion mutant

pMMB207-F

CTCCCGTTCTGGATAATGTT

For complemented mutants

pMMB207-R

GGCGTTTCACTTCTGAGTTCG

For complemented mutants

pMMB207-dam-F

AGCTCGGTACCCGGGGATCCTCTAGCTAAAGGAAGACGTTATGAAAAAAAATCGCGCTTTTTTGA

For complemented mutant of ∆dam

pMMB207-dam-R

TCTCATCCGCCAAAACAGCCAAGCTTTATTTTCTTGCAGGCGTTGCGACT

For complemented mutant of ∆dam

pMMB207-invC-F

AGCTCGGTACCCGGGGATCCTCTAGCTAAAGGAAGACGTTATGAAAACACCTCGTTTACTGCAAT

For complemented mutant of ∆ invC

pMMB207-invC-R

TCTCATCCGCCAAAACAGCCAAGCTTTAATTCTGGTCAGCGAATGCATTC

For complemented mutant of ∆ invC

pMMB207-prgH-F

AGCTCGGTACCCGGGGATCCTCTAGCTAAAGGAAGACGTTATGGAAACATCAAAAGAGAAGACGA

For complemented mutant of ∆prgH

pMMB207-prgH-R

TCTCATCCGCCAAAACAGCCAAGCTTTAAAGTGGGCTTGGGAAATACCAA

For complemented mutant of ∆prgH

pMMB207-spaN-F

AGCTCGGTACCCGGGGATCCTCTAGCTAAAGGAAGACGTTATGGGCGATGTGTCAGCTGTCAGTT

For complemented mutant of ∆spaN

pMMB207-spaN-R

TCTCATCCGCCAAAACAGCCAAGCTTCAGGCGTCATCCTCCTCGCCAGAT

For complemented mutant of ∆spaN

pCX340-F

AGACAATCTGTGTGGGCACTCGACC

For β-lactamase TEM-1 fusion plasmid

pCX340-R

TTCTGAGAATAGTGTATGCGGCGAC

For β-lactamase TEM-1 fusion plasmid

pCX340-dam-F

AAGGAGGAATAACATATGATGAAAAAAAATCGCGCTTTTTTGA

For β-lactamase TEM-1 fusion plasmid

pCX340-dam-R

GTGCGAATTCTCCGCGGAGGTACCTTTTCTTGCAGGCGTTGCGACT

For β-lactamase TEM-1 fusion plasmid

pCX340-invC-F

AAGGAGGAATAACATATGATGAAAACACCTCGTTTACTGCAAT

For β-lactamase TEM-1 fusion plasmid

pCX340-invC-R

GTGCGAATTCTCCGCGGAGGTACCATTCTGGTCAGCGAATGCATTC

For β-lactamase TEM-1 fusion plasmid

pCX340-prgH-F

AAGGAGGAATAACATATGATGGAAACATCAAAAGAGAAGACGA

For β-lactamase TEM-1 fusion plasmid

pCX340-prgH-R

GTGCGAATTCTCCGCGGAGGTACCAAGTGGGCTTGGGAAATACCAA

For β-lactamase TEM-1 fusion plasmid

pCX340-spaN-F

AAGGAGGAATAACATATGATGGGCGATGTGTCAGCTGTCAGTT

For β-lactamase TEM-1 fusion plasmid

pCX340-spaN-R

GTGCGAATTCTCCGCGGAGGTACCGGCGTCATCCTCCTCGCCAGAT

For β-lactamase TEM-1 fusion plasmid