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Table 1 Bacterial strains and plasmids used in this study

From: Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation

Strain or plasmid Relevant characteristics Reference
Escherichia coli
 X7213 λpir Host for π requiring plasmids, conjugal donor Labortaory collection
 X7213 λpir-pSC189 X7213 λpir with pSC189, Kmr, Cmr This study
SalmonellaEnteritidis
 C50336 Wild type Obtained from Chinese National Institute for the Control of Pharmaceutical and Biological
 C50336∆dam C50336, In-frame deletion in dam This study
 C50336∆invC C50336, In-frame deletion in invC This study
 C50336∆hilD C50336, In-frame deletion in hilD This study
 C50336∆prgH C50336, In-frame deletion in prgH This study
 C50336∆spaN C50336, In-frame deletion in spaN This study
 C50336∆dam::dam C50336∆dam with pMMB207 expressing the dam gene,Cmr This study
 C50336∆ invC::invC C50336∆ invC with pMMB207 expressing the invC gene,Cmr This study
 C50336∆hilD::hilD C50336∆ invC with pMMB207 expressing the hilD gene,Cmr This study
 C50336∆ prgH::prgH C50336∆ prgH with pMMB207 expressing the prgH gene,Cmr This study
 C50336∆ spaN::spaN C50336∆dam with pMMB207 expressing the dam gene,Cmr This study
 C50336-pMMB207 C50336 with pMMB207,Cmr This study
 C50336::dam C50336 with pMMB207 expressing the dam gene,Cmr This study
 C50336::prgH C50336 with pMMB207 expressing the prgH gene,Cmr This study
 C50336∆dam-pMMB207 C50336∆dam with pMMB207,Cmr This study
 C50336∆prgH-pMMB207 C50336∆prgH with pMMB207,Cmr This study
 C50336-pCX340 C50336 with pCX340, Tetr This study
 C50336-pCX340-dam C50336 with pCX340 expressing the dam gene, Tetr This study
 C50336-pCX340-invC C50336 with pCX340 expressing the invC gene, Tetr This study
 C50336-pCX340-prgH C50336 with pCX340 expressing the prgH gene, Tetr This study
 C50336-pCX340-spaN C50336 with pCX340 expressing the dam gene, Tetr This study
Plasmids
 pSC189 Transposon delivery vector, R6K, Kmr, Cmr [74]
 pDM4 Suicide vector, pir dependent, R6K, SacBR, Cmr [75]
 pMMB207 IncQ lacIq Δbla Ptac-laclacZa, Cmr [24]
 pMMB207-dam pMMB207 derivative containing dam, Cmr This study
 pMMB207-invC pMMB207 derivative containing invC, Cmr This study
 pMMB207-hilD pMMB207 derivative containing hilD, Cmr This study
 pMMB207-prgH pMMB207 derivative containing prgH, Cmr This study
 pMMB207-spaN pMMB207 derivative containing spaN, Cmr This study
 pCX340 pBR322 derivative, cloning vector used to fuse effectors to TEM-1-β-lactamase, Tetr [76]
 pCX340-dam pCX340 derivative containing dam This study
 pCX340-invC pCX340 derivative containing invC This study
 pCX340-prgH pCX340 derivative containing prgH This study
 pCX340-spaN pCX340 derivative containing spaN This study
  1. The antibiotics as follows: kanamycin (Kmr), 100 μg/mL; chloramphenicol (Cmr), 25 μg/mL; tetracycline (Tetr), 12.5 μg/mL
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