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Fig. 9 | BMC Microbiology

Fig. 9

From: Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation

Fig. 9

qRT-PCR validation of expression levels of the overlapping DEGs from MAPK signaling pathway a, TGF-β signaling pathway b, TNF signaling pathway c, and FoxO signaling pathway d in the C50336-VS-C50336Δdam group. J774A.1 cells were pre-treated with LPS (1 μg/mL, 5 h) and then infected with C50336 or C50336Δdam at an MOI of 20 for 4 h. Each group comprised three independent replicates. The cells were then collected, and the total RNA was isolated for qRT-PCR. The mRNA expression level was normalized against the mouse GAPDH transcript. Data are presented as mean ± SEM of log2 (fold change) of triplicate samples

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