Fig. 5

Wogonin attenuated HSV-stimulated NF-κB activation. a HEC-1-A cells were transfected with an NF-κB-luc reporter plasmid. The cells were mock-treated or treated with the indicated concentrations of wogonin or MG132 (5 μg/ml) prior to mock infection or infection with HSV-2 (MOI = 1). The relative luciferase activity was determined after 24 h. The data are presented the mean values (±SDs) of triplicate determinations from three dependent experiments. b Wogonin prevented virus-induced IκB-α degradation. HEC-1-A cells were mock-infected or infected with HSV-2 (MOI = 1) in the absence or presence of wogonin (50 and 100 μM) or MG132 (5 μg/ml). The IκB-α levels were visualized 24 h p.i. by western blot analysis. c Wogonin reversed HSV-2-induced p65 nuclear translocation. HEC-1-A cells were mock-infected or infected with HSV-2 (MOI = 1) in the presence or absence of wogonin (100 μM). The p65 translocation was determined via immunofluorescence assay 24 h p.i