Fig. 4

Wogonin inhibited HSV IE gene expression. a to b) HEC-1-A cells were either mock-treated or treated with wogonin (100 μM) and then infected with HSV-1 (MOI = 1). The protein expression levels of ICP4, ICP0 (A) and ICP27 b at each time point were determined via western blot analysis and normalized to those of GAPDH. c Wogonin inhibited HSV-1/blue ICP4 promoter-driven lacZ gene expression in a dose-dependent manner. HEC-1-A cells were pretreated with serial concentrations of wogonin 30 min prior to infection with HSV-1/blue (MOI = 1). The β-Gal activity was measured as described 12 h p.i. The data are presented as the mean values (±SDs) of triplicate determinations from three dependent experiments