Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method

Table 3 MIC against different plant pathogens and hemolysis percentage displayed by the peptide conjugates derived from BP100 tested in this study

Peptide type	Code	MIC (μM)			Hemolysis^d (%)
Peptide type	Code	*Xav*^a	*Psa*^b	Ea^c	50 μM	150 μM	250 μM
Reference peptides	tag54	> 100	> 100	> 100	0	0	1
Reference peptides	BP100	10–20	7.5–10	7.5–10	1	8	18
BP100 (dimer)	BP192	7.5–10	7.5–10	7.5–10	51	69	69
	BP198	10–20	10–20	10–20	59	72	72
	BP213	1.25–2.5	2.5–5.0	2.5–5.0	90	92	98
BP100 - Melittin [10,11,12,13,14,15,16,17,18,19]	BP170	1.25–2.5	2.5–5.0	2.5–5.0	82	93	98
BP100 - Melittin [10,11,12,13,14,15,16,17,18,19]	BP171	2.5–5.0	1.25–2.5	2.5–5.0	5	16	34
BP100 - Magainin [4,5,6,7,8,9,10]	BP180	2.5–5.0	2.5–5.0	2.5–5.0	12	54	58
BP100 - Magainin [4,5,6,7,8,9,10]	BP181	2.5–5.0	1.25–2.5	2.5–5.0	0	0	0
BP100 - Magainin [1,2,3,4,5,6,7,8,9,10]	BP175	1.25–2.5	2.5–5.0	5.0–7.5	6	14	32
	BP176	2.5–5.0	2.5–5.0	5.0–7.5	3	44	59
	BP178	2.5–5.0	2.5–5.0	2.5–5.0	0	3	25
BP100 - Cecropin A [25,26,27,28,29,30,31,32,33,34,35,36,37]	BP188	2.5–5.0	1.25–2.5	2.5–5.0	10	24	42

^aXav, Xanthomonas axonopodis pv. vesicatoria; ^bPsa, Pseudomonas syringae pv. syringae; ^cEa, Erwinia amylovora; ^dPercent hemolysis plus confidence interval (α = 0.05)

ISSN: 1471-2180