Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method

Table 2 Screening of conjugate peptides derived from BP100 against Xff, compared with BP100 and tag54, by means of a contact test combined with v-qPCR method

Peptide type	Code	Sequence	N° of AA	Log N₀/N^a
Peptide type	Code	Sequence	N° of AA	3.1 μM		12.5 μM
Reference peptides	tag54	KDWEHLKDWEHLKDWEHL-OH	18	0 ± 0	g	0 ± 0	G
Reference peptides	BP100	KKLFKKILKYL-NH₂	11	1.39 ± 0.07	d	3.27 ± 0.09	ABC
BP100 (dimer)	BP192	KKLFKKILKYL - AGPA - KKLFKKILKYL - KDEL-OH	30	0.91 ± 0.13	e	1.33 ± 0.11	F
	BP198	KKLFKKILKYL - KKLFKKILKYL - KDEL-OH	26	1.37 ± 0.04	d	1.94 ± 0.08	DE
	BP213	KKLFKKILKYL - AGPA - LYKLIKKFLKK - KDEL - OH	30	1.17 ± 0.02	de	1.34 ± 0.10	F
BP100 - Melittin [10,11,12,13,14,15,16,17,18,19]	BP170	KKLFKKILKYL - TTGLPALISW - OH	21	2.89 ± 0.09	ab	3.11 ± 0.06	BC
BP100 - Melittin [10,11,12,13,14,15,16,17,18,19]	BP171	KKLFKKILKYL - AGPA - TTGLPALISW-OH	25	2.91 ± 0.02	ab	3.79 ± 0.15	A
BP100 - Magainin [4,5,6,7,8,9,10]	BP180	KKLFKKILKYL - KFLHSAK-OH	18	2.29 ± 0.27	c	2.96 ± 0.19	C
BP100 - Magainin [4,5,6,7,8,9,10]	BP181	KKLFKKILKYL - AGPA - KFLHSAK-OH	22	1.30 ± 0.08	de	2.40 ± 0.37	D
BP100 - Magainin [1,2,3,4,5,6,7,8,9,10]	BP175	KKLFKKILKYL - AGPA - GIGKFLHSAK-OH	25	2.65 ± 0.18	abc	3.52 ± 0.16	AB
	BP176	KKLFKKILKYL - GIGKFLHSAK-OH	21	2.47 ± 0.12	bc	3.34 ± 0.11	ABC
	BP178	KKLFKKILKYL - AGPA - GIGKFLHSAK - KDEL-OH	29	2.95 ± 0.22	a	3.54 ± 0.05	AB
BP100 - Cecropin A [25,26,27,28,29,30,31,32,33,34,35,36,37]	BP188	KKLFKKILKYL - AVAVVGQATQIAK - KDEL-OH	28	0.89 ± 0.04	e	1.60 ± 0.09	EF

^aLog reduction of Xff cell viability after the treatment with the peptides (at 3.1 and 12.5 μM) for 3 h was calculated as log N₀/N, where N₀ is the initial number of viable cells and N is the number of viable cells after the treatment, as estimated by v-qPCR. Values are the mean of three replicates, the confidence intervals are indicated. Lowercase letters correspond to the means comparison of Log N₀/N in 3.1 μM. Capital letters correspond to the means comparison of Log N₀/N in 12.5 μM. Means sharing the same letter indicate no significant differences between peptides (P < 0.05), according to the Tukey’s test

ISSN: 1471-2180