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Table 2 Screening of conjugate peptides derived from BP100 against Xff, compared with BP100 and tag54, by means of a contact test combined with v-qPCR method

From: Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method

Peptide type Code Sequence N° of AA Log N0/Na    
3.1 μM   12.5 μM  
Reference peptides tag54 KDWEHLKDWEHLKDWEHL-OH 18 0 ± 0 g 0 ± 0 G
BP100 KKLFKKILKYL-NH2 11 1.39 ± 0.07 d 3.27 ± 0.09 ABC
BP100 (dimer) BP192 KKLFKKILKYL - AGPA - KKLFKKILKYL - KDEL-OH 30 0.91 ± 0.13 e 1.33 ± 0.11 F
BP198 KKLFKKILKYL - KKLFKKILKYL - KDEL-OH 26 1.37 ± 0.04 d 1.94 ± 0.08 DE
BP213 KKLFKKILKYL - AGPA - LYKLIKKFLKK - KDEL - OH 30 1.17 ± 0.02 de 1.34 ± 0.10 F
BP100 - Melittin [10,11,12,13,14,15,16,17,18,19] BP170 KKLFKKILKYL - TTGLPALISW - OH 21 2.89 ± 0.09 ab 3.11 ± 0.06 BC
BP171 KKLFKKILKYL - AGPA - TTGLPALISW-OH 25 2.91 ± 0.02 ab 3.79 ± 0.15 A
BP100 - Magainin [4,5,6,7,8,9,10] BP180 KKLFKKILKYL - KFLHSAK-OH 18 2.29 ± 0.27 c 2.96 ± 0.19 C
BP181 KKLFKKILKYL - AGPA - KFLHSAK-OH 22 1.30 ± 0.08 de 2.40 ± 0.37 D
BP100 - Magainin [1,2,3,4,5,6,7,8,9,10] BP175 KKLFKKILKYL - AGPA - GIGKFLHSAK-OH 25 2.65 ± 0.18 abc 3.52 ± 0.16 AB
BP176 KKLFKKILKYL - GIGKFLHSAK-OH 21 2.47 ± 0.12 bc 3.34 ± 0.11 ABC
BP178 KKLFKKILKYL - AGPA - GIGKFLHSAK - KDEL-OH 29 2.95 ± 0.22 a 3.54 ± 0.05 AB
BP100 - Cecropin A [25,26,27,28,29,30,31,32,33,34,35,36,37] BP188 KKLFKKILKYL - AVAVVGQATQIAK - KDEL-OH 28 0.89 ± 0.04 e 1.60 ± 0.09 EF
  1. aLog reduction of Xff cell viability after the treatment with the peptides (at 3.1 and 12.5 μM) for 3 h was calculated as log N0/N, where N0 is the initial number of viable cells and N is the number of viable cells after the treatment, as estimated by v-qPCR. Values are the mean of three replicates, the confidence intervals are indicated. Lowercase letters correspond to the means comparison of Log N0/N in 3.1 μM. Capital letters correspond to the means comparison of Log N0/N in 12.5 μM. Means sharing the same letter indicate no significant differences between peptides (P < 0.05), according to the Tukey’s test
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