Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method

Table 1 Primers and TaqMan probes used for qPCR analysis, amplification efficiency and sensitivity analysis

qPCR assay	Primer/ probe	Sequence	Amplicon length (bp)	Slope	R²	Efficiency (%)	Sensitivity^a	Reference of source
HL-1	rev-2	GGTTTTGCTGACTGGCAACA	221	−3.47	0.99	94	30.8	37
HL-2	rev-3	CACTTGTGGTAAGCATCCTGAG	307	−3.49	0.99	94	31.8	This study
	for	AAGGCAATAAACGCGCACTA						37
	probe	FAM/−TGGCAGGCAGCAACGATACGGCT−/BHQ						37
XF16S-1	rev-1	CCGATGTATTCCTCACCCGTC	62	−3.39	0.99	97	27.2	39
XF16S-2	rev-2	CTAATCGGACATCGGCTCAT	181	−3.39	0.99	97	28.1	This study
XF16S-3	rev-3	GTAGGAGTCTGGACCGTGTCTC	279	−3.39	0.99	97	29.7	21
	for	CGGCAGCACGTTGGTAGTAA						39
	probe	FAM/−CATGGGTGGCGAGTGGC−/TAMRA						39
EFTu-1	rev-1	GGCGAGCCAACAAAATGTGTT	77	−3.21	0.99	95	28.4	38
EFTu-2	rev-2	ATCACCAGGAAAATCATACTTGCT	202	−3.38	0.99	98	29.4	This study
EFTu-3	rev-3	GAATGTGGGTATCCAATGCTTC	311	−3.21	0.99	95	33.9	This study
	for	GGATGGTGCGATTTTAGTATGTTCT						38
	probe	FAM/−TGATGGTCCGATGCCTCAGACTCGT−/TAMRA						38

ISSN: 1471-2180