Correlation of drug resistance with single nucleotide variations through genome analysis and experimental validation in a multi-drug resistant clinical isolate of M. tuberculosis

Table 2 Distribution of stop-loss variation in VPCI591

Genomic Position	Gene Id	Gene Name	Nucleotide Change	Stop codon changing to Amino Acid	Function	AA added	Predicted function^a
392,261	Rv0325	none	T223C	X75Q	Hypothetical protein	155	Class I SAM-dependent methyltransferase
715,266	Rv0621	none	A1065G	X355W	Possible membrane protein	31
932,280	Rv0836c	none	A653G	X218W	Hypothetical protein	23
1,315,191	Rv1180	pks3	A1467C	X489Y	Probable polyketide beta-ketoacyl synthase Pks3	1597	Msl3 (mass like protein3)
1,694,547	Rv1504c	none	T598G	X200E	Hypothetical protein	182
2,020,563	Rv1783	none	A1307T	X436L	ESX-5 type VII secretion system protein	15
2,052,687	Rv1809	PPE33	G1406C	X469S	PPE protein	1
2,120,796	Rv1870c	none	A635T	X212L	Hypothetical protein	11	Endonuclease
3,007,238	Rv2690c	none	T1972C	X658R	Integral membrane alanine and valine and leucine rich protein	6
3,788,365	Rv3373	echA18	T640G	X214G	Probable enoyl-CoA hydratase EchA18	83
4,190,285	Rv3739c	PPE67	A233G	X78W	PPE67	18
4,383,655	Rv3898c	none	T331C	X111Q	Hypothetical protein	215

^aPredicted function of the longer protein resulting from loss of stop codon due to SNV

ISSN: 1471-2180