Correlation of drug resistance with single nucleotide variations through genome analysis and experimental validation in a multi-drug resistant clinical isolate of M. tuberculosis

Table 1 Distribution of stop-gain variation in VPCI591

Genomic Position	Gene Id	Gene Name	Nucleotide Change	Amino acid changing to stop codon	No. AA lost	Function	Domains lost
162,226	Rv0134	ephF	G456A	W152X	148	Possible epoxide hydrolase EphF	Hydrolase domain
234,477	Rv0197	Rv0197	T2247G	Y749X	14	Possible oxidoreductase
309,765	Rv0257	Rv0257	C67T	R23X	102	Hypothetical protein
549,251	Rv0457c	Rv0457c	G357A	W119X	555	Probable peptidase	Oligopeptidase domain
704,997	Rv0610c	Rv0610c	C913T	Q305X	81	Hypothetical protein
1,009,490	Rv0906	Rv0906	C547T	Q183X	190	Hypothetical protein
1,037,911	Rv0930	pstA1	C913T	R305X	3	Probable phosphate-transport integral membrane ABC transporter PstA1
2,399,782	Rv2139	pyrD	C1063T	Q355X	2	Probable dihydroorotate dehydrogenase
2,448,288	Rv2187	fadD15	G129A	W43X	558	Actelyco asynthetase like	AMP synthetase ligase
2,882,317	Rv2563	Rv2563	C28T	Q10X	340	Probable glutamine-transport transmembrane ABC transporter	Cytoplasmic domain
2,929,354	Rv2601	speE	C967T	Q323X	202	Spermidine synthatase
2,936,497	Rv2608	PPE42	C1452A	Y484X	97	PPE family protein PPE42	PPE C-terminal domain
3,097,349	Rv2788	sirR	C391T	Q131X	130	Probable transcriptional repressor SirR
3,351,472	Rv2994	Rv2994	G204A	W68X	377	Probable integral membrane protein
3,378,720	Rv3019c	esxR	G282A	W94X	3	Secreted ESAT-6 like protein EsxR
3,442,240	Rv3079c	Rv3079c	G358T	E120X	156	Hypothetical protein	Luciferase like domain
3,689,523	Rv3303c	lpdA	C1416A	C472X	22	NAD(P) H quinone reductase LpdA
4,351,039	Rv3872	PE35	G295T	E99X	1	PE family-related protein PE35

ISSN: 1471-2180