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Fig. 3 | BMC Microbiology

Fig. 3

From: A survey of RNA viruses in mosquitoes from Mozambique reveals novel genetic lineages of flaviviruses and phenuiviruses, as well as frequent flavivirus-like viral DNA forms in Mansonia

Fig. 3

a Phylogenetic analysis of partial flavivirus NS5 nucleotide sequences (0.5 kbp DNA amplicon) from insect-specific flaviviruses. cISF and dISF indicate classical and dual-host associated insect-specific flaviviruses, respectively. At specific branches, the number of “*” indicates the support revealed by the different phylogenetic reconstructions methods used (bootstrap values ≥75% and posterior probability values ≥0.80 were assumed as significant). The sequences described in this work are indicated in bold-face, and their origin (mosquito species) is also indicated. All the sequences used are designated by their respective accession numbers|virus name. The size bar indicates the number of nucleotide substitutions per site. b amplification of flavivirus-like from RNA and mosquito genomic DNA using a combination of treatments that included the use/or not (+/) of DNase I followed/or not (+/−) by reverse transcription (RT). The NZYtech ladder VI was used as a molecular mass marker. c Structure of the flavivirus wild-type and the NS5 fragments analyzed. The 3′-end of the viral genome is schematically shown at the top. §-Mosquito species could not be confirmed by COI sequence

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BMC Microbiology

ISSN: 1471-2180

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