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Fig. 1 | BMC Microbiology

Fig. 1

From: Total RNA and genomic DNA of Lactobacillus gasseri OLL2809 induce interleukin-12 production in the mouse macrophage cell line J774.1 via toll-like receptors 7 and 9

Fig. 1

Confocal microscopic observation (a) and flow cytometric analysis (b) of J774.1 cells that phagocytosed L. gasseri OLL2809. After cultivation for 24 h in the presence of fluorescein-4-isothiocyanate (FITC)-labelled L. gasseri OLL2809 (green), J774.1 cells were labelled with Cell Mask™ Deep Red (red) and observed under a confocal microscope (a). The right and below panel showed x-z and y-z stacks at the yellow lines. After incubation for 48 h in the absence (black) or in the presence of fluorescein-4-isothiocyanate (FITC)-labelled L. gasseri OLL2809 (red), the J774.1 cells were labelled with allophycocyanin conjugated anti-F4/80 antibody and analysed by flow cytometry (b)

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BMC Microbiology

ISSN: 1471-2180

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