Table 4 Primers used to confirm the presence of E. coli O157:H7 and virulence genes
Target gene | Primer name | Primer sequence (5′–3′) | Amplification size (bp) |
|---|---|---|---|
rfbE | O157AF | AAGATTGCGCTGAAGCCTTTG | 497 |
| Â | O157AR | CATTGGCATCGTGTGGACAG | |
fliC | FLICH7 F | GCGCTGTCGAGTTCTATCGAGC | 625 |
| Â | FLICH7 R | CAACGGTGACTTTATCGCCATTCC | |
uidA | PT-2 | GCGAAAACTGTGGAATTGGG | 252 |
PT-3 | TGATGCTCCATAACTTCCTG | ||
hlyA | hlyA F | GCATCATCAAGCGTACGTTCC | 534 |
hlyA R | AATGAGCCAAGCTGGTTAAGCT | ||
stx2 | Stx2F | GGCACTGTCTGAAACTGCTCC | 255 |
Stx2R | TCGCCAGTTATCTGACATTCTG | ||
stx1 | Stx1F | ATAAATCGCCATTCGTTGACTAC | 180 |
Stx1R | AGAACGCCCACTGAGATCATC |