Fig. 2
Expression dynamics of T3SS apparatus encoding genes of NGJ1. a RT-PCR based expression analysis of T3SS apparatus encoding genes of NGJ1 during mycophagous interaction with R. solani in PDB. The differential gene expression was estimated during mycophagy with respect to the expression observed during bacterial growth in absence of fungi. b RT-PCR based expression analysis of T3SS apparatus encoding genes of NGJ1 in PDB with or without EGTA/CaCl2 supplementation. The differential gene expression was calculated in presence of EGTA/CaCl2 with respect to that observed in absence of EGTA/CaCl2. The 16S rRNA gene of NGJ1 was used as endogenous control in RT-PCR analysis. The experiments were independently repeated three times with a minimum three technical replicates. Asterisks * indicate significant difference at P < 0.05 (estimated using one-way ANOVA). Graphs show mean values ± standard deviation