Fig. 1

Isolating non-growing cell subpopulations with ampicillin-induced cell lysing and protein-dilution methods. mCherry positive cells from overnight (24 h) pre-cultures were diluted 100-fold in fresh LB broth without IPTG. Upon reaching the exponential-growth phase (OD₆₀₀ = 0.25), cells were treated with ampicillin at 10X MIC concentration (60 μg/ml). Growing cell, non-growing cell and dead-cell/debris subpopulations are highlighted with dark green, red and orange circles, respectively. Red Fl.: Red Fluorescence