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Fig. 4 | BMC Microbiology

Fig. 4

From: Antimicrobial and immunomodulatory responses of photodynamic therapy in Galleria mellonella model

Fig. 4

ALA-PDT improves hemocyte density and susceptibility to pathogens. Hemocytes were taken and pooled from five larvae for each experiment group. a Quantification of G. mellonella hemocytes. ALA-PDT, G. mellonella received ALA-mediated PDT treatment. Control, G. mellonella were injected with PBS. ***, p < 0.001. b Hemocytes were stimulated with S. aureus (MOI = 2) for 3 h (right). Arrow indicated the morphological changes of hemocyte after stimulation of S. aureus. Scale bar, 50 μm. c Staining of presentative hemocytes. Larvae were pretreated with ALA-PDT for 4 h. Hemocytes were collected and incubated with S. aureus (MOI = 2) for 3 h. DAPI (300 nM) labeled the nuclear of hemocytes and WGA (10 μg/mL) labeled S. aureus cell wall. Scale bar, 50 μm. d The aggregation of G. mellonella hemocytes to conidia of C. albicans. Hemocytes collected from G. mellonella with receiving ALA-PDT treatment were inoculated with C. albicans (MOI = 2) for 2 h. Arrow, C. albicans conidia. Scale bar, 50 μm

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